蒿甲醚对日本血吸虫葡萄糖摄取及糖原含量的影响

Effect of artemether on glucose uptake and glycogen content in Schistosoma japonicum.

作者信息

Xiao S H, You J Q, Mei J Y, Guo H F, Jiao P Y, Sun H L, Yao M Y, Feng Z

机构信息

Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, China.

出版信息

Zhongguo Yao Li Xue Bao. 1997 Jul;18(4):363-7.

PMID:10072924

Abstract

AIM

To study the effect of artemether (Art) on glucose uptake and glycogen content in schistosomes.

METHODS

Schistosomes recovered from mice treated intragastrically with Art 300 mg.kg-1 for 24-48 h, were incubated in the drug-free medium containing [U-14C]glucose 11.1 MBq.L-1. The glycogen content, [U-14C]glucose uptake, and incorporation of [U-14C]glucose into worm glycogen in both male and female worms were determined.

RESULTS

When above-mentioned schistosomes were exposed to drug-free medium containing [U-14C]glucose for 1-24 h, the glycogen contents of male and female worms decreased 27%-61% and 39%-78%, respectively. Only 3 out of 6 male worm groups showed 23%-35% decrease in glucose uptake, while much less glucose uptake was found in female worms in all groups with reduction rates of 18%-38%. Apart from 2 male groups no apparent change in the incorporation of [U-14C]glucose into the worm glycogen was seen.

CONCLUSIONS

Art-induced glycogen reduction in schistosomes was related to an inhibition of glycolysis rather than an interference with glucose uptake.

摘要

目的

研究蒿甲醚（Art）对血吸虫葡萄糖摄取及糖原含量的影响。

方法

从经300mg·kg-1蒿甲醚灌胃处理24 - 48小时的小鼠体内获取血吸虫，将其置于含有11.1MBq·L-1 [U-14C]葡萄糖的无药培养基中孵育。测定雄虫和雌虫的糖原含量、[U-14C]葡萄糖摄取量以及[U-14C]葡萄糖掺入虫体糖原的情况。

结果

当上述血吸虫在含有[U-14C]葡萄糖的无药培养基中暴露1 - 24小时后，雄虫和雌虫的糖原含量分别下降了27% - 61%和39% - 78%。6个雄虫组中只有3组葡萄糖摄取量下降了23% - 35%，而所有雌虫组的葡萄糖摄取量下降幅度更小，为18% - 38%。除2个雄虫组外，[U-14C]葡萄糖掺入虫体糖原未见明显变化。