Jiang L, Wen H, Ito A
National Hydatid Disease Centre of China, Xinjiang Institute for Endemic Diseases Control and Research, 141 Jianquan Road, Urumqi 830002, Xinjiang, China.
Trans R Soc Trop Med Hyg. 2001 May-Jun;95(3):285-8. doi: 10.1016/s0035-9203(01)90235-4.
Immunoblotting analysis was carried out using crude extracts of Echinococccus multilocularis and E. granulosus. A total of 214 serum samples were examined for the detection of antibody responses against 18-kDa polypeptides (Eg18 and Em18). Of 44 sera from patients with alveolar echinococcosis (AE) 91% were positive against both Eg18 and Em18, compared with 10% and 13%, respectively, of 70 cystic echinococcosis (CE) samples and 13% and 17% of 29 cysticercosis samples. A relatively purified 18-kDa antigen was extracted from E. granulosus protoscoleces and an 18-kDa-ELISA test which is simple, fast and highly sensitive and specific has been established. A comparative ELISA analysis, using the purified 18-kDa antigen and pooled AE and CE serum, indicated that there are significant differences in antibody levels between AE and CE sera. The maximum ratio for the evaluation of AE to CE was 2.26. The 18-kDa-ELISA was positive for 91% (40/44) and 11% (8/70) of AE and CE sera, respectively, and there was no cross-reactivity with cysticercosis sera or healthy controls. The overall diagnostic values of 18-kDa-ELISA for AE sera were sensitivity 91%, specificity 94%, positive predictive value 83% and negative predictive value 97%. We conclude that (i) the 18-kDa antigenic component may not be species specific but (ii) levels of antibody to the 18-kDa antigen are significantly different between AE and CE sera. Therefore, the 18-kDa antigen can be a reliable serological marker for differentiation of AE from CE.
使用多房棘球绦虫和细粒棘球绦虫的粗提物进行免疫印迹分析。共检测了214份血清样本，以检测针对18 kDa多肽（Eg18和Em18）的抗体反应。肺泡型棘球蚴病（AE）患者的44份血清中，91%对Eg18和Em18均呈阳性，相比之下，囊型棘球蚴病（CE）的70份样本分别为10%和13%，囊尾蚴病的29份样本分别为13%和17%。从细粒棘球绦虫原头节中提取了相对纯化的18 kDa抗原，并建立了一种简单、快速、高度灵敏且特异的18 kDa-ELISA检测方法。使用纯化的18 kDa抗原以及AE和CE混合血清进行的比较ELISA分析表明，AE和CE血清之间的抗体水平存在显著差异。评估AE与CE的最大比值为2.26。18 kDa-ELISA检测中，AE血清的阳性率为91%（40/44），CE血清的阳性率为11%（8/70），与囊尾蚴病血清或健康对照无交叉反应。18 kDa-ELISA对AE血清的总体诊断价值为：敏感性91%，特异性94%，阳性预测值83%，阴性预测值97%。我们得出结论：（i）18 kDa抗原成分可能不是种特异性的，但（ii）AE和CE血清中针对18 kDa抗原的抗体水平存在显著差异。因此，18 kDa抗原可作为区分AE和CE的可靠血清学标志物。
