十二指肠钩口线虫和美洲板口线虫细胞色素C氧化酶1基因的测序
[Sequencing of cytochrome C oxidase 1 gene of Ancylostoma duodenale and Necator americanus].
作者信息
Li T, Zhan B, Hawdon J M, Gong X, Xiao S, Shan Q, Feng Z, Hotez P J
机构信息
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):81-3.
AIM
To identify the genetic diversity between Ancylostoma duodenale and Necator americanus.
METHODS
Mitochondrial cytochrome C oxidase subunit 1 (CO1) gene was amplified from genomic DNA of human hookworms collected from infected patients in Hejiang County, Sichuan Province, and the purified PCR products were directly sequenced by using Licor auto-sequencer.
RESULTS
The PCR products were about 700 bp. Alignment of CO1 gene fragment sequences showed 89.7% similarity between Ancylostoma duodenale and Necator americanus, but still certain nucleotide variations (10.3%) existed.
CONCLUSION
CO1 gene sequence can be used as a marker to identify the two species of human hookworms.
目的
鉴定十二指肠钩口线虫和美洲板口线虫之间的遗传多样性。
方法
从四川省合江县感染患者体内采集的人体钩虫基因组DNA中扩增线粒体细胞色素C氧化酶亚基1（CO1）基因，纯化后的PCR产物使用Licor自动测序仪直接测序。
结果
PCR产物约700 bp。CO1基因片段序列比对显示，十二指肠钩口线虫和美洲板口线虫之间的相似性为89.7%，但仍存在一定的核苷酸变异（10.3%）。
结论
CO1基因序列可作为鉴定两种人体钩虫的标志物。
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