[中国5个省份采集的美洲板口线虫细胞色素C氧化酶1（COI）基因多态性分析]
[Polymorphism analysis of cytochrome C oxidase 1 (COI) gene in Necator americanus collected from 5 provinces in China].
作者信息
Li Tie-hua, Guo Xiang-rong, Hu Ling, Xiao Shu-hua, Xue Hai-chou, Hawdon John
机构信息
Institute of Parasitic Diseases, Chinese Centre for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(4):210-3.
OBJECTIVE
To detect and compare the COI gene sequences of Necator americanus collected from the provinces of Sichuan, Hainan, Yunnan, Hubei and Jiangsu, and to analyze the genetic diversity of the geographic isolates.
METHODS
COI genes of N. americanus were amplified from the genomic DNA by PCR and sequenced.
RESULTS
The COI gene sequences of N. americanus from five provinces were 97%-99% identical over 595 bp, and base variation occurred in 19 nucleotide sites in which the transition was more frequent than the transversion. The difference between the sequences ranged from 1.34% to 2.18%.
CONCLUSION
The COI gene sequences show high identity among the geographic isolates of N. americanus with some difference at specific nucleotide sites.
目的
检测并比较从四川、海南、云南、湖北和江苏等省份采集的美洲板口线虫的细胞色素氧化酶亚基I（COI）基因序列，并分析这些地理分离株的遗传多样性。
方法
通过聚合酶链反应（PCR）从基因组DNA中扩增美洲板口线虫的COI基因并进行测序。
结果
五个省份的美洲板口线虫的COI基因序列在595 bp上的同源性为97%-99%，在19个核苷酸位点发生碱基变异，其中转换比颠换更频繁。序列之间的差异范围为1.34%至2.18%。
结论
美洲板口线虫的地理分离株之间的COI基因序列显示出高度同源性，但在特定核苷酸位点存在一些差异。
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