Wang S, Zheng H, Tao Z, Piessens W F
Guizhou Provincial Institute of Parasitic Diseases, Guiyang 550001.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):90-4.
To determine whether immunization with recombinant filarial chitinase or a fragment containing the epitope recognized by McAbMF1 and SXP-1 could protect jirds against microfilaremia resulting from infection with B. malayi.
Test jirds were immunized with the following recombinant parasite antigens: filarial chitinase, the c-terminal fragments F7R2 or F8R2 of r-chitinase, filarial SXP-1, myosin or maltose binding protein (MBP). Employing immunochemical techniqe (SDS-PAGE, Western Blotting) and serology (ELISA) measured antifilarial antibodies level.
Immunization of jirds with recombinant chitinase induced partial protection against microfilaremia resulting from subsequent infection with B. malayi, but did not reduce adult worm burdens. Vaccination was much less effective when administered during the prepatent stage of infection and was ineffective when given to microfilaremic jirds. Immunization of jirds with SXP-1, an antigen present in multiple worm stages also reduced microfilaremia and, in some experiments, adult worm burdens.
The recombinant chitinase, fragments F7R2 and F8R2 and SXP-1 could provide partial protection against microfilaremia in jirds.
确定用重组丝虫几丁质酶或含有被单克隆抗体 MF1 和 SXP - 1 识别的表位的片段进行免疫是否能保护沙鼠免受马来布鲁线虫感染导致的微丝蚴血症。
用以下重组寄生虫抗原来免疫实验沙鼠：丝虫几丁质酶、重组几丁质酶的 c 末端片段 F7R2 或 F8R2、丝虫 SXP - 1、肌球蛋白或麦芽糖结合蛋白（MBP）。采用免疫化学技术（SDS - PAGE、蛋白质印迹法）和血清学（ELISA）来检测抗丝虫抗体水平。
用重组几丁质酶免疫沙鼠可诱导对随后马来布鲁线虫感染导致的微丝蚴血症产生部分保护作用，但不能减少成虫负荷。在感染的潜伏期进行疫苗接种效果要差得多，而对有微丝蚴血症的沙鼠进行接种则无效。用 SXP - 1（一种在多个虫期都存在的抗原）免疫沙鼠也可减少微丝蚴血症，并且在一些实验中还可减少成虫负荷。
重组几丁质酶、片段 F7R2 和 F8R2 以及 SXP - 1 可为沙鼠的微丝蚴血症提供部分保护。
