Deng S, Hu Y
Department of Pharmacology, Third Military Medical University, Chongqing 400038.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(5):282-4.
To study the mechanism of antimalarial action of nitroquine.
Intraerythrocytic P. yoelii was cultured by the method of Trager and Jensen. The amount of [3H]-ethanolamine incorporation was measured as an index of the phospholipid synthesis. DPH was used as a probe to measure the plasmodial fluorescent polarization.
The incorporation of [3H]-ethanolamine into the P. yoelii infected erthrocytes was markedly inhibited by nitroquine. The plasmodial membrane polarization and viscosity were significantly increased by nitroquine.
Nitroquine could inhibit the phospholipid synthesis and decrease the membrane fluidity of P. yoelii.
研究硝喹抗疟作用的机制。
采用Trager和Jensen的方法培养红细胞内约氏疟原虫。测定[3H]-乙醇胺掺入量作为磷脂合成的指标。用DPH作为探针测量疟原虫的荧光偏振。
硝喹显著抑制[3H]-乙醇胺掺入约氏疟原虫感染的红细胞。硝喹使疟原虫膜的偏振和黏度显著增加。
硝喹可抑制约氏疟原虫的磷脂合成并降低其膜流动性。
