Chen X H, Hu Y M
Department of Pharmacology, Third Military Medical College, Chongqing, China.
Zhongguo Yao Li Xue Bao. 1993 Nov;14 Suppl:S37-40.
Erythrocytes of mice infected with Plasmodium yoelii of early stage (ESP) were separated from those infected with late stage (LSP), by a single density-gradient centrifugation in 19% diatrizoate meglumine. After centrifugation, the differential counts of ESP and LSP were 84.9% and 87.4%, respectively. The stage-dependent effects of nitroquine (NQ), pyrimethamine (Pyr), perphenazine (Per) and metronidazole (Met) were studied with [3H]adenosine incorporation into ESP and LSP in vitro using chloroquine (CQ) as control. Like CQ, the 4 drugs inhibited that [3H]adenosine incorporation into LSP more remarkably than that into ESP. The IC50 of CQ, Met, Pyr, Per, and NQ against ESP were 1.89, 2.11, 2.56, 2.60, and 4.65 times as high as that against LSP, respectively.
通过在19%泛影葡胺中进行单次密度梯度离心，将感染约氏疟原虫早期（ESP）的小鼠红细胞与感染晚期（LSP）的小鼠红细胞分离。离心后，ESP和LSP的鉴别计数分别为84.9%和87.4%。以氯喹（CQ）为对照，体外研究了硝喹（NQ）、乙胺嘧啶（Pyr）、奋乃静（Per）和甲硝唑（Met）对ESP和LSP的阶段依赖性影响，采用[3H]腺苷掺入法。与CQ一样，这4种药物对LSP中[3H]腺苷掺入的抑制作用比对ESP的抑制作用更显著。CQ、Met、Pyr、Per和NQ对ESP的IC50分别是对LSP的IC50的1.89、2.11、2.56、2.60和4.65倍。
