Jiang G, Liu R, Daubenberger C A, Pluschke G
Department of Medical Parasitology, Guangdong College of Pharmacy, Guangzhou 510224.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(5):294-7.
To obtain the complete sequence and analyze the diversity of the MSP 1 molecule from the Chinese isolates of Plasmodium falciparum.
Genomic DNA was prepared directly from blood samples spotted on filter papers from 2 malaria patients from Baoting County, Hainan Province. PCR amplification of the target gene was carried out using 5 pairs of oligonucleotides specific for the MSP 1 gene. Direct sequencing of the target gene fragments was performed using ABI PRISM Dye Terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer) in a automatic ABI PRISM 310 Genetic Analyzer.
For the first time, two complete sequences of the MSP1 gene from two Chinese isolates of Plasmodium falciparum were obtained. A comparison with the previously reported sequences identified them as members of the MAD20 allelic family. The deduced amino acid sequences of the MSP1 from this two Chinese isolates were identical with each other except for Blocks 2, 4 and 8.
The sequences of the MSP 1 from two Chinese isolates of P. falciparum belong to the MAD20 allelic family. Minor variations through the whole sequences exist compared with the MAD20 sequence. The results provide the first evidence of the diversity of the MSP 1 molecule from Chinese isolates of Plasmodium falciparum.
获取恶性疟原虫中国分离株MSP 1分子的完整序列并分析其多样性。
直接从海南省保亭县2例疟疾患者滤纸血样中提取基因组DNA。使用5对针对MSP 1基因的寡核苷酸对目标基因进行PCR扩增。使用ABI PRISM Dye Terminator Cycle Sequencing Ready Reaction Kit（Perkin Elmer）在自动ABI PRISM 310遗传分析仪上对目标基因片段进行直接测序。
首次获得了两株恶性疟原虫中国分离株的MSP1基因完整序列。与先前报道的序列比较，确定它们为MAD20等位基因家族成员。这两株中国分离株MSP1推导的氨基酸序列除第2、4和8区外彼此相同。
两株恶性疟原虫中国分离株的MSP 1序列属于MAD20等位基因家族。与MAD20序列相比，整个序列存在微小变异。结果首次提供了恶性疟原虫中国分离株MSP 1分子多样性的证据。
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