Zhu X, Zhou L, Liu Q, Gao X
Department of Parasitology, Capital University of Medical Sciences, Beijing 100054.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(3):155-8.
To identify the genotype of merozoite surface protein 1 of Plasmodium falciparum in Yunnan Province and explore the polymorphism of MSP1 genes in geographical characteristics and genetics.
Nested polymerase chain reaction was applied to genotyping of P. falciparum isolated in Yunnan Province. Some of parasite alleles were sequenced by dye primer cycle sequencing.
In 30 P. falciparum infections, 38 different alleles were found. Of them, the dominant allele was a variant of MAD20, while was K1 less and the RO33 was few. In addition, incidences of mixed allele infections were observed. Sequence analysis showed that DNA sequences of MAD20, K1 and RO33 alleles from Yunnan were highly homologous with those of international standard strains, respectively.
Composition and sequence characteristics of P. falciparum arasite population in the endemic area can be detected by genotyping with MSP1 as genetic marker, which would be useful for the prevention and treatment of malaria.
鉴定云南省恶性疟原虫裂殖子表面蛋白1的基因型，探讨MSP1基因在地理特征和遗传学方面的多态性。
采用巢式聚合酶链反应对云南省分离的恶性疟原虫进行基因分型。部分寄生虫等位基因通过染料引物循环测序进行测序。
在30例恶性疟原虫感染中，发现38种不同的等位基因。其中，优势等位基因为MAD20的一个变体，K1较少，RO33更少。此外，观察到混合等位基因感染的发生率。序列分析表明，云南的MAD20、K1和RO33等位基因的DNA序列分别与国际标准菌株的序列高度同源。
以MSP1为遗传标记进行基因分型可检测流行区恶性疟原虫种群的组成和序列特征，这对疟疾的防治具有重要意义。
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