Hommel Mirja, Kyewski Bruno
Tumor Immunology Program, Division of Cellular Immunology, German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany.
J Exp Med. 2003 Feb 3;197(3):269-80. doi: 10.1084/jem.20021512.
Activation of antigen-specific T cells by mature dendritic cells in secondary lymphoid organs is a key control point of the adaptive immune response. Here we describe the ex vivo isolation of preformed multicellular clusters between T cells and antigen-presenting cells. Adoptively transferred, antigen-specific T cells segregated into individual clusters where their activation and proliferation was initiated in vivo. Transit of the T cell cohort through the cluster compartment required 32-36 h. The precise timing of the response to agonistic epitopes was remarkably invariant regardless of the T cell lineage, the major histocompatibility complex haplotype, and the antigen dose. Interestingly, initiation of cell division of T cells specific for a subdominant epitope and a weak agonist was delayed by 6 h. The results provide a basis for the analysis of short range, mutual cell-cell interactions within such confined microenvironments.
在二级淋巴器官中，成熟树突状细胞激活抗原特异性T细胞是适应性免疫反应的关键控制点。在此，我们描述了T细胞与抗原呈递细胞之间预先形成的多细胞簇的体外分离。过继转移的抗原特异性T细胞分离成单个簇，在体内启动其激活和增殖。T细胞群体通过簇隔室需要32 - 36小时。无论T细胞谱系、主要组织相容性复合体单倍型和抗原剂量如何，对激动性表位反应的精确时间都非常恒定。有趣的是，针对亚优势表位和弱激动剂的T细胞的细胞分裂起始延迟了6小时。这些结果为分析这种受限微环境内的短程相互细胞间相互作用提供了基础。
