[不同地区微小按蚊核糖体DNA第二内部转录间隔区的序列差异]
[Sequence difference of ribosomal DNA second internal transcribed spacer in Anopheles minimus in different localities].
作者信息
Zhou Shui-sen, Tang Lin-hua, Gu Zheng-cheng, Wang Yi
机构信息
Institute for Parasitic Disease Control and Prevention, Chinese Center for Disease Control and Prenention, Shanghai 200025.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(1):29-31.
OBJECTIVE
To compare the sequence difference of the ribosomal DNA second internal transcribed spacer (ITS2) in Anopheles minimus from Yunnan, Hainan, Guangxi of China and from Thailand.
METHODS
Single mosquito's legs were digested to extract DNA; the rDNA-ITS2 region was amplified by specific primers and the PCR products were purified, sequenced and analysed.
RESULTS
Two different kinds of ITS2 sequences, temporarily named as An. minimus A and An. minimus C, were found in this study. The length and GC contents of ITS2 region in species A and species C are 481 bp, 54.04% and 483 bp, 54.23%, respectively. Two species differed by 5.8% in the ITS2 sequences owing to 22 fixed nucleotide substitutions and indels.
CONCLUSION
Two cryptic species, An. minimus A and An. minimus C, were found in the research localities.
目的
比较中国云南、海南、广西以及泰国微小按蚊核糖体DNA第二内部转录间隔区（ITS2）的序列差异。
方法
消化单只蚊子的腿部以提取DNA；用特异性引物扩增rDNA-ITS2区域，对PCR产物进行纯化、测序及分析。
结果
本研究发现了两种不同类型的ITS2序列，暂命名为微小按蚊A和微小按蚊C。A种和C种ITS2区域的长度和GC含量分别为481 bp、54.04%和483 bp、54.23%。由于22个固定的核苷酸替换和插入/缺失，两种按蚊的ITS2序列差异为5.8%。
结论
在研究地点发现了两个隐种，即微小按蚊A和微小按蚊C。
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