Chen Ying-dan, Lin Bao-ying, Zhang Jia-xun
Institute for Parasitic Disease Control and Prevention, Chinese Center for Disease Control and Prenention, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(1):37-8.
To induce a line of Plasmodium berghei with resistance to artemisinin.
The major methods included blood transmission from passage to passage and progressive increase of drug pressure.
The resistant lines were developed by different protocols: (A) The initial dosage of artemisinin was 126.2 mg/kg which was increased by 60 mg/kg for the next passage and boosted by 126.2 mg/kg for every other passage. As developed to passage 60 and 76, the resistant index was 18.39:1 and 14.89:1 respectively, then decreased gradually. For passage 108, the dosage was 8,862.5 mg/kg, but the resistant index was only 10.49:1. (B) Using passage 66 from (A) as the source, a dosage of 4,000 mg/kg was given each week, the resistance of the passage 40 increased significantly with an index of 27.5:1. (C) Using passage 19 of (B) as the source, drug was administered at the dose of 2,000 mg/kg each week. The resistant index of passage 15 was 17.41:1.
Line of P. berghei with medium level resistance to artemisinin was established.
诱导出对青蒿素具有抗性的伯氏疟原虫品系。
主要方法包括逐代血液传代和逐步增加药物压力。
通过不同方案培育出抗性品系：(A)青蒿素初始剂量为126.2mg/kg，下一代增加60mg/kg，每隔一代增加126.2mg/kg。培育至第60代和第76代时，抗性指数分别为18.39:1和14.89:1，随后逐渐下降。至第108代时，剂量为8862.5mg/kg，但抗性指数仅为10.49:1。(B)以(A)中的第66代作为起始，每周给予4000mg/kg的剂量，第40代的抗性显著增加，指数为27.5:1。(C)以(B)中的第19代作为起始，每周给予2000mg/kg的药物。第15代的抗性指数为17.41:1。
建立了对青蒿素具有中等水平抗性的伯氏疟原虫品系。
