全氟萘烷对兔角膜作用的实验研究

Lu F, Tang S, Zhu X, Zhang J, Zheng H

Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.

Yan Ke Xue Bao. 2001 Mar;17(1):16-20.

PURPOSE

To study the effects of perfluorodecalin on the cornea of the rabbit eyes.

METHODS

Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endothelial cells were monitored clinically by slit-lamp biomicroscope and specular microscope for 26 weeks. Animals were sacrificed in 1st, 2nd, 4th, 10th, 16th, 22nd, and 26th week after injection, respectively, and the corneas were examined under the light microscope.

RESULTS

Perfluorodecalin droplets looking like "fish eggs" were found at about 1/4-1/2 of the corneal height in the inferior anterior chamber. Corneal opacification on the area contacted with perfluorodecalin was observed in five eyes five weeks after injection, and all in the 22nd week. Mutton fat KPs in one eye were seen in the 6th week firstly, and in all eyes in the 7th week. Corneal pannus formation in one eye was present in the 4th week, two eyes in the 5th week and three eyes in the 6th week. Retrocorneal fibrous membrane in one eye was detected at the 6th week and 3 eyes at the 7th week respectively. After injection of perfluorodecalin, endothelial cell density was significantly decreased (2,789 +/- 192 vs. 2,341 +/- 658, P < 0.01) and corneal thickness was increased.

CONCLUSIONS

Perfluorodecalin injected into anterior chamber can lead to corneal damage and inflammatory reaction.

目的

研究全氟萘烷对兔眼角膜的影响。

方法

将全氟萘烷（0.05 ml/只）注入18只兔眼的前房。通过裂隙灯生物显微镜和角膜内皮显微镜临床监测角膜形态和内皮细胞，持续26周。分别在注射后的第1、2、4、10、16、22和26周处死动物，取角膜在光学显微镜下检查。

结果

在下眼前房约角膜高度的1/4 - 1/2处发现形似“鱼卵”的全氟萘烷液滴。注射后5周，5只眼中观察到与全氟萘烷接触区域的角膜浑浊，至第22周时所有眼均出现。6周时1只眼中首次发现羊脂状角膜后沉着物（KPs），第7周时所有眼中均出现。4周时1只眼出现角膜血管翳形成，5周时2只眼出现，6周时3只眼出现。分别在第6周和第7周检测到1只眼和3只眼出现角膜后纤维膜。注射全氟萘烷后，内皮细胞密度显著降低（2789±192对2341±658，P < 0.01），角膜厚度增加。