Ding X, Li C, Lu L, Feng G, Zheng H
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou 510060, China.
Yan Ke Xue Bao. 2001 Mar;17(1):21-6.
To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes.
Fifteen New Zealand white rabbits were divided into two groups: experimental group(F6H8) and control group(BSS). All rabbits underwent anterior chamber injection of 0.15 ml F6H8 or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre-operatively and postoperatively. Histopathological examination and Transmission electron microscopy(TEM) were done after the rabbits were sacrificed.
All the corneas were clear. Since 4 weeks after operation, the endothelial cells were markedly irregular in size and shape and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes(RCM) grew gradually 2 weeks after surgery. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of plasma were seen in TEM.
Corneal endothelial cell degenerated after contacting with F6H8 for 2-4 weeks. As a silicone solvent, it should be removed completely after injection. We don't recommend it to be used as a new intraocular temponade.
研究全氟己基辛烷（F6H8）对兔眼角膜内皮细胞（CEC）的影响。
将15只新西兰白兔分为两组：实验组（F6H8）和对照组（平衡盐溶液，BSS）。所有兔子均行前房注射0.15 ml F6H8或BSS。术前及术后行裂隙灯生物显微镜检查及角膜内皮照相。兔子处死后进行组织病理学检查及透射电子显微镜（TEM）检查。
所有角膜均保持透明。术后4周起，内皮细胞大小和形状明显不规则，内皮细胞数量明显减少。术后2周起后弹力层膜（RCM）逐渐生长增厚。部分内皮细胞可见空泡变性。TEM检查可见细胞核变性及细胞质水肿。
角膜内皮细胞与F6H8接触2 - 4周后发生变性。作为一种硅油溶剂，注射后应彻底清除。不推荐将其用作新型眼内填充剂。
