Deng X G, Li J C, Zhu L
Henan Institute of Ophthalmology, Zhengzhou 450003.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(5):301-4.
To find a rapid method for diagnosing Acanthamoeba keratitis and identifying Acanthamoeba.
10% potassium hydroxide (KOH) wet-mount preparations, Acanthamoeba culture, inverted phase contrast microscopy, and pathological examination using H. E. staining and PAS staining.
Using corneal scrapings and corneal materials obtained from surgery, 7 cases and 5 cases of Acanthamoeba keratitis were diagnosed by 10% KOH wet-mount preparations. 6 strains of Acanthamoeba were isolated in corneal materials of 6 cases by protozoa culture method. The cysts, trophozoites and pseudopods on the trophozoites of Acanthamoeba were directly observed under the inverted phase contrast microscope. The cysts and trophozoites of Acanthamoeba were seen by H. E. staining and PAS staining with 20 h.
Acanthamoeba keratitis could be rapidly diagnosed by 10% KOH wet-mount preparations and inverted phase contrast microscopy. Acanthamoeba organisms could be directly observed and identified under inverted phase contrast microscope.
寻找一种快速诊断棘阿米巴角膜炎并鉴定棘阿米巴的方法。
10%氢氧化钾（KOH）湿片法、棘阿米巴培养、倒置相差显微镜检查以及苏木精-伊红（H.E.）染色和过碘酸雪夫（PAS）染色的病理检查。
利用角膜刮片和手术获取的角膜材料，10% KOH湿片法诊断出7例和5例棘阿米巴角膜炎。通过原虫培养法从6例的角膜材料中分离出6株棘阿米巴。在倒置相差显微镜下直接观察到棘阿米巴的包囊、滋养体以及滋养体上的伪足。苏木精-伊红染色和过碘酸雪夫染色20小时后可见棘阿米巴的包囊和滋养体。
10% KOH湿片法和倒置相差显微镜检查可快速诊断棘阿米巴角膜炎。在倒置相差显微镜下可直接观察并鉴定棘阿米巴生物体。
