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本文引用的文献

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Epidemiological, clinical and laboratory findings of infectious keratitis at Mansoura Ophthalmic Center, Egypt.埃及曼苏拉眼科中心感染性角膜炎的流行病学、临床及实验室检查结果
Int J Ophthalmol. 2017 Jan 18;10(1):61-67. doi: 10.18240/ijo.2017.01.10. eCollection 2017.
2
Experiences and Factors that Influence Potassium Hydroxide Examination by Microscopists.显微镜检查人员进行氢氧化钾检查的经验及影响因素。
Med Mycol J. 2016;57(3):E29-34. doi: 10.3314/mmj.15-00022.
3
Spectrum and Sensitivity of Bacterial Keratitis Isolates in Auckland.奥克兰细菌性角膜炎分离株的谱系及敏感性
J Ophthalmol. 2016;2016:3769341. doi: 10.1155/2016/3769341. Epub 2016 Apr 26.
4
Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience.真菌性角膜炎的分子诊断评估。十年经验。
J Ophthalmic Inflamm Infect. 2011 Feb 23;1(1):15-22. doi: 10.1007/s12348-011-0019-9.
5
Etiologic diagnosis of corneal ulceration at a tertiary eye center in Kathmandu, Nepal.尼泊尔加德满都一家三级眼科中心角膜溃疡的病因学诊断。
Cornea. 2010 Dec;29(12):1380-5. doi: 10.1097/ICO.0b013e3181d92881.
6
Review of epidemiological features, microbiological diagnosis and treatment outcome of microbial keratitis: experience of over a decade.微生物性角膜炎的流行病学特征、微生物学诊断和治疗结果回顾：十余年经验。
Indian J Ophthalmol. 2009 Jul-Aug;57(4):273-9. doi: 10.4103/0301-4738.53051.
7
Microbial keratitis in the developing world: does prevention work?发展中国家的微生物性角膜炎：预防措施是否有效？
Int Ophthalmol Clin. 2007 Summer;47(3):17-25. doi: 10.1097/IIO.0b013e318074e0b3.
8
Microbiological diagnosis of infective keratitis: comparative evaluation of direct microscopy and culture results.感染性角膜炎的微生物学诊断：直接显微镜检查与培养结果的比较评估
Br J Ophthalmol. 2006 Oct;90(10):1271-6. doi: 10.1136/bjo.2006.096230. Epub 2006 Jul 12.
9
Laboratory diagnosis in ulcerative keratitis.溃疡性角膜炎的实验室诊断
Ophthalmic Res. 2005 May-Jun;37(3):123-7. doi: 10.1159/000084273. Epub 2005 Mar 4.
10
Spectrum of fungal keratitis in North India.印度北部真菌性角膜炎的谱系
Cornea. 2005 Jan;24(1):8-15. doi: 10.1097/01.ico.0000126435.25751.20.

在一家三级护理中心中，角膜炎患者的角膜刮片的阳性微生物染色：一项描述性的横断面研究。

Positive Microbiological Stains of Corneal Scrapings among Patients with Keratitis in a Tertiary Care Centre: A Descriptive Cross-sectional Study.

机构信息

Department of Ophthalmic Pathology and Laboratory Medicine, Biratnagar Eye Hospital, Rani, Biratnagar, Morang, Nepal.

Department of Cornea, Biratnagar Eye Hospital, Rani, Biratnagar, Morang, Nepal.

出版信息

JNMA J Nepal Med Assoc. 2022 Jun 1;60(250):546-550. doi: 10.31729/jnma.7538.

DOI:10.31729/jnma.7538
PMID:35690971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9275455/
Abstract

INTRODUCTION

Keratitis, an ocular emergency, requires rapid and accurate treatment to prevent vision impairment. Wet mount direct microscopy examination of corneal scraping smear using gram and 10% potassium hydroxide stain helps in early diagnosis and treatment. The main objective of this study was to find out the prevalence of positive microbiological stains of corneal scrapings among patients with keratitis in a tertiary care centre.

METHODS

A descriptive cross-sectional study was conducted in the Department of Ophthalmic Pathology and Laboratory Medicine from January, 2018 to December, 2019. Data collection was done after taking ethical approval from the Institutional Review Committee of the hospital (Reference number: BEH-IRC-35/A). All corneal smear samples received in this department were included in this study. Case records with incomplete data were excluded. Whole sampling was done. The data were analyzed using Statistical Package for the Social Sciences version 22.0. Frequency and percentage was calculated for binary data.

RESULTS

Among 4631 corneal scrapings, microbiological stains were positive in 3538 (76.40%) patients.

CONCLUSIONS

The prevalence of positive microbiological stains of corneal scrapings in our study was higher in comparison to other studies done in similar settings. This technique could be used where culture facilities are unavailable or unaffordable.

KEYWORDS

keratitis; microscopy; Nepal.

摘要

简介

角膜炎是一种眼部急症，需要快速准确的治疗以防止视力受损。使用革兰氏染色和 10%氢氧化钾染色对角膜刮片进行湿片直接显微镜检查有助于早期诊断和治疗。本研究的主要目的是找出在一家三级保健中心就诊的角膜炎患者中角膜刮片的微生物染色阳性率。

方法

这是一项在眼科病理与实验室医学科进行的描述性横断面研究，时间从 2018 年 1 月至 2019 年 12 月。在获得医院机构审查委员会的伦理批准（参考编号：BEH-IRC-35/A）后进行数据收集。本研究纳入了该科室收到的所有角膜涂片样本。排除了数据不完整的病历。采用整群抽样方法。使用社会科学统计软件包 22.0 对数据进行分析。对二项数据进行了频率和百分比计算。

结果

在 4631 例角膜刮片中，有 3538 例（76.40%）患者的微生物染色呈阳性。

结论

与在类似环境中进行的其他研究相比，我们的研究中角膜刮片微生物染色阳性率更高。在培养设施不可用或无法负担的情况下，可以使用这种技术。

关键词

角膜炎；显微镜检查；尼泊尔