Xu L, Chen J, Hung T
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, 510060 China.
Yan Ke Xue Bao. 2001 Jun;17(2):68-71.
To evaluate the role of cryopreserved corneas on penetrating keratoplasty (PKP) by comparing them with fresh corneas on clinical application.
Third-six excellent condition patients were chosen and randomly divided into two groups. They were performed PKP by using fresh and cryopreserved corneas respectively. The graft transparency, epithelial healing speed of graft, tear membrane, graft thickness, endothelium density and visual acurity were emphasized respectively.
When fresh and cryopreserved corneas were used on PKP as the donor respectively, postoperative time to recover normal break-up time (BUT) was two to six months average (4.7 +/- 0.3) months and two to eight months, average (5.6 +/- 0.4) months and time to recover normal Schirmer's test was one to four months average (2.1 +/- 0.3) months and 1-3.5 months, average (1.7 +/- 0.5) months. The graft's thickness, stabilized visual acurity and graft's rejective rate were 0.56-0.68 mm (average 0.62 mm) vs 0.54-0.62 mm (average 0.59 mm), (0.46 +/- 0.03) vs (0.44 +/- 0.05) and 20% vs 19% respectively when fresh vs cryoperserved corneas. No statistical significance between fresh and cryopreserved corneas (P > 0.05). But the grafts behaved intact epithelium and continuing transparency when fresh corneas as donors, while the graft's epithelial defects healed after three to five days of the operation and edematous grafts were reclear after two to three weeks of the operation when cryopreserved corneas as donors. Graft's endothelium density in fresh corneas group 2,043-2,210 cells/mm2 (average 2,135 cells/mm2) was high than in cryopreserved corneas 1,240-1,860 cells/mm2 (average 1,672 cells/mm2) (P < 0.01).
There were no statistical significance on the transparent rate of grafts, grafts thickness, visual acurity and graft's rejective rate between fresh and cryopreserved corneas. But graft's epithelium healing was delayed and endothelium density was lower when cryopreserved corneas as donors.
通过在临床应用中比较新鲜角膜和冷冻保存角膜，评估冷冻保存角膜在穿透性角膜移植术（PKP）中的作用。
选取36例病情良好的患者，随机分为两组。分别使用新鲜角膜和冷冻保存角膜进行PKP。分别重点观察植片透明度、植片上皮愈合速度、泪膜、植片厚度、内皮细胞密度和视力。
当分别使用新鲜角膜和冷冻保存角膜作为供体进行PKP时，术后恢复正常泪膜破裂时间（BUT）的时间平均为2至6个月（4.7±0.3）个月和2至8个月，平均（5.6±0.4）个月，恢复正常泪液分泌试验的时间平均为1至4个月（2.1±0.3）个月和1至3.5个月，平均（1.7±0.5）个月。新鲜角膜与冷冻保存角膜相比，植片厚度、稳定视力和植片排斥率分别为0.56 - 0.68mm（平均0.62mm）对0.54 - 0.62mm（平均0.59mm），（0.46±0.03）对（0.44±0.05），以及20%对19%。新鲜角膜和冷冻保存角膜之间无统计学意义（P>0.05）。但是，当使用新鲜角膜作为供体时，植片上皮完整且持续透明，而当使用冷冻保存角膜作为供体时，植片上皮缺损在术后3至5天愈合，水肿的植片在术后2至3周重新变透明。新鲜角膜组植片内皮细胞密度为2,043 - 2,210个细胞/mm²（平均2,135个细胞/mm²）高于冷冻保存角膜的1,240 - 1,860个细胞/mm²（平均1,672个细胞/mm²）（P<0.01）。
新鲜角膜和冷冻保存角膜在植片透明率、植片厚度、视力和植片排斥率方面无统计学意义。但是，当使用冷冻保存角膜作为供体时，植片上皮愈合延迟且内皮细胞密度较低。
