Chen Shao-hong, Chang Zheng-shan, Chen Ming-gang, Zhang Yong-nian, Feng Zheng
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(3):155-7.
To improve Paragonimus westermani chromosome preparation technique.
After being exposed to colchicine, the gonadal cells of P. westermani were treated by the following procedures: hypotonicity, fixation, dropping onto a slide and staining.
The chromosome number of P. westermani is 22, and the karyotype is 2m + 6Sm + 14t chromosome.
The improved technique of chromosome preparation is feasible to operate and the chromosome is clear enough for karyotype analysis.
改进卫氏并殖吸虫染色体标本制备技术。
对卫氏并殖吸虫性腺细胞进行秋水仙素处理后，依次进行低渗、固定、滴片及染色等操作。
卫氏并殖吸虫染色体数目为22条，核型为2m + 6Sm + 14t染色体。
改进后的染色体标本制备技术操作可行，染色体清晰，可用于核型分析。
