[卫氏并殖吸虫染色体核型分析标本制备方法的改进]
[Improvement in the preparation of Paragonimus westermani chromosome for karyotype analysis].
作者信息
Chen Shao-hong, Chang Zheng-shan, Chen Ming-gang, Zhang Yong-nian, Feng Zheng
机构信息
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(3):155-7.
OBJECTIVE
To improve Paragonimus westermani chromosome preparation technique.
METHODS
After being exposed to colchicine, the gonadal cells of P. westermani were treated by the following procedures: hypotonicity, fixation, dropping onto a slide and staining.
RESULTS
The chromosome number of P. westermani is 22, and the karyotype is 2m + 6Sm + 14t chromosome.
CONCLUSION
The improved technique of chromosome preparation is feasible to operate and the chromosome is clear enough for karyotype analysis.
目的
改进卫氏并殖吸虫染色体标本制备技术。
方法
对卫氏并殖吸虫性腺细胞进行秋水仙素处理后，依次进行低渗、固定、滴片及染色等操作。
结果
卫氏并殖吸虫染色体数目为22条，核型为2m + 6Sm + 14t染色体。
结论
改进后的染色体标本制备技术操作可行，染色体清晰，可用于核型分析。
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