Hirai H, Sakaguchi Y, Habe S, Imai H T
Z Parasitenkd. 1985;71(5):617-29. doi: 10.1007/BF00925595.
We examined C-banded karyotypes of six species of lung flukes from Japan and Korea; diploid and triploid Paragonimus westermani, P. miyazakii, P. ohirai, P. iloktsuenensis and P. sadoensis, with special reference to their karyotypic diversification. C-band analysis between the diploid and the triploid westermani revealed that two of three homologues of the triploid resembled those of the diploid in C-band pattern, while the remaining chromosome showed a different pattern from any species examined here. This karyological evidence indicates that the triploid is allotriploid probably induced by interspecific hybridization between the diploid westermani and an unknown species; we, therefore, suggest that the triploid westermani is an independent species and synonymous with P. pulmonalis (Miyazaki 1978). As the morphologically similar three species, ohirai, iloktsuenensis and sadoensis, had the same C-band polymorphism in chromosome No. 4, these species are classified as the local races of P. ohirai. Paragonimus miyazakii has one common C-band (5q) with the diploid westermani, but other bands (1q, 4q, 6q, 7p and 7q) are different. From these observations, the six species examined are phylogenetically divided into three groups: (1) westermani group containing diploid and triploid (= pulmonalis) species, (2) miyazakii and (3) ohirai including two geographic races, iloktsuenensis and sadoensis.
我们检查了来自日本和韩国的六种肺吸虫的C带核型；二倍体和三倍体的卫氏并殖吸虫、宫崎并殖吸虫、大平并殖吸虫、伊洛并殖吸虫和佐渡并殖吸虫，特别关注它们的核型多样化。二倍体和三倍体卫氏并殖吸虫之间的C带分析表明，三倍体的三个同源染色体中有两个在C带模式上与二倍体相似，而其余染色体显示出与这里检查的任何物种都不同的模式。这种核型学证据表明，三倍体可能是由二倍体卫氏并殖吸虫与一个未知物种之间的种间杂交诱导产生的异源三倍体；因此，我们认为三倍体卫氏并殖吸虫是一个独立的物种，与肺并殖吸虫同义（宫崎，1978年）。由于形态相似的大平并殖吸虫、伊洛并殖吸虫和佐渡并殖吸虫在第4号染色体上具有相同的C带多态性，这些物种被归类为大平并殖吸虫的地方种群。宫崎并殖吸虫与二倍体卫氏并殖吸虫有一个共同的C带（5q），但其他带（1q、4q、6q、7p和7q）不同。从这些观察结果来看，所检查的六个物种在系统发育上分为三组：（1）包括二倍体和三倍体（=肺并殖吸虫）物种的卫氏并殖吸虫组，（2）宫崎并殖吸虫组，（3）包括伊洛并殖吸虫和佐渡并殖吸虫两个地理种群的大平并殖吸虫组。
