Hu Ya-lan, Guo Shu-zhong, Yan Pei-song
Plastic Surgery Center, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P. R. China 710032.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2002 Sep;16(5):340-4.
To investigate the effect of local application of basic fibroblast growth factor (bFGF) and sucralfate on the skin tissue structure after expansion.
Continuous tissue expansion (CTE) experimental animal model was made with constant pressure pump in nine white pigs. The bFGF and sucralfate were perfused between tissue and tissue expander. Both bFGF and sucralfate were injected in group I, both bFGF and normal saline in group II, only sucralfate in group III, and normal saline in group IV as control group. The samples were took from each pig for histomorphological assessment on the 3rd day and 6th week after expansion.
Compared with control group, epidermal, granular spinous and basal cell thickness increased significantly (P < 0.05) in group I; in dermal layer, dermal thickened slightly and the collagen fibers became thicker and orderly; the elastic fiber regenerated significantly; the density of fibroblasts and capillaries significantly increased (P < 0.05) in group I. The collagen fibers were broken on the 3rd day and 6th week after expansion in control group. However, the thickness of the fibrous capsule layer was similar in each group.
Local application of exogenous bFGF and sucralfate combined with CTE can greatly promote the skin growth. It can accelerate the tissue expansion.
探讨局部应用碱性成纤维细胞生长因子（bFGF）和硫糖铝对扩张后皮肤组织结构的影响。
用恒压泵对9只白色猪制作连续组织扩张（CTE）实验动物模型。将bFGF和硫糖铝灌注于组织与组织扩张器之间。I组注射bFGF和硫糖铝，II组注射bFGF和生理盐水，III组仅注射硫糖铝，IV组注射生理盐水作为对照组。在扩张后第3天和第6周从每只猪身上取材进行组织形态学评估。
与对照组相比，I组表皮、颗粒层、棘层和基底细胞厚度显著增加（P<0.05）；在真皮层，真皮轻度增厚，胶原纤维变粗且排列有序；弹性纤维显著再生；I组成纤维细胞和毛细血管密度显著增加（P<0.05）。对照组在扩张后第3天和第6周胶原纤维断裂。然而，各组纤维囊层厚度相似。
外源性bFGF和硫糖铝局部应用联合CTE可极大促进皮肤生长。可加速组织扩张。
