Jacobs M M, Ward D N, Griffin A C
Proc Soc Exp Biol Med. 1976 Mar;151(3):568-70. doi: 10.3181/00379727-151-39260.
Porcine follicle-stimulating hormone (pFSH) has been prepared from acetone dried pituitary glands. The acetone powder was initially extracted in pH 5 ethanol-acetate buffer (40% ethanol and acetate at 0.5 ionic strength) containing 0.02% PMSF. The gonadotropin rich fraction, obtained in the 80% ethanol precipitate, was dissolved in 0.12 M NH4HCO3, heated at 60 degrees for 3 min, cooled, and centrifuged. The supernatant was lyophilized for chromatography on QAE-Sephadex, then Sephadex G-100. The pFSH obtained has a biological activity of 15 X NIH-FSH-P-1 and 2.1% sialic acid. The amino acid analysis is characterized by high lysine, aspartic acid, and glutamic acid with notable amounts of threonine, proline, and half-cystine.
猪促卵泡激素（pFSH）是从丙酮干燥的垂体中制备的。丙酮粉最初在含有0.02%苯甲基磺酰氟（PMSF）的pH 5乙醇 - 醋酸盐缓冲液（40%乙醇，醋酸盐浓度为0.5离子强度）中提取。在80%乙醇沉淀中获得的富含促性腺激素的部分，溶解于0.12 M碳酸氢铵中，在60℃加热3分钟，冷却后离心。上清液冻干后用于在QAE - 葡聚糖凝胶上进行色谱分析，然后在葡聚糖凝胶G - 100上进行分析。所获得的pFSH具有15倍美国国立卫生研究院促卵泡激素标准品P - 1（NIH - FSH - P - 1）的生物活性，且含有2.1%的唾液酸。氨基酸分析的特征是赖氨酸、天冬氨酸和谷氨酸含量高，同时含有大量的苏氨酸、脯氨酸和半胱氨酸。
