Whitley R J, Keutmann H T, Ryan R J
Endocrinology. 1978 Jun;102(6):1874-86. doi: 10.1210/endo-102-6-1874.
Methods are described for isolating highly purified FSH from porcine pituitary glands. The biological activity of pure material was 22 NIH-FSH-P2 U/mg, as judged by the ovarian weight augmentation bioassay. Virtually no immunoreactive LH was indicated by RIA, and only one component was detected by both gel electrophoresis and immunoprecipitation. Amino acid and carbohydrate analyses of the highly purified FSH indicated the presence of 1-tryptophan, a high content of aspartic and glutamic acids, and 6% sialic acid. Molecular weights of untreated and guanidine-treated porcine FSH were estimated from hydrodynamic measurements of Stokes radii and sedimentation coefficients. A highly specific and sensitive RIA was also developed for this hormone.
描述了从猪垂体中分离高纯度促卵泡激素（FSH）的方法。通过卵巢增重生物测定法判断，纯物质的生物活性为22 NIH-FSH-P2 U/mg。放射免疫分析（RIA）显示几乎没有免疫反应性促黄体生成素（LH），并且通过凝胶电泳和免疫沉淀仅检测到一个成分。对高纯度FSH的氨基酸和碳水化合物分析表明存在1-色氨酸、高含量的天冬氨酸和谷氨酸以及6%的唾液酸。从未经处理和经胍处理的猪FSH的斯托克斯半径和沉降系数的流体动力学测量估计其分子量。还针对这种激素开发了一种高度特异性和灵敏的RIA。
