Anderegg Tamara R, Jones Ronald N
The JONES Group/JMI Laboratories, North Liberty, Iowa, USA.
Diagn Microbiol Infect Dis. 2003 Jan;45(1):73-6. doi: 10.1016/s0732-8893(02)00482-0.
AZD2563 is a new, long-acting oxazolidinone that possesses potent antimicrobial activity against Gram-positive organisms. This report summarizes the results of broth microdilution MIC and disk diffusion quality control (QC) investigations for AZD2563. All tests or methods (M2-A7 and M7-A5) and QC study designs (M23-A2) were those published by the National Committee for Clinical Laboratory Standards. QC strains tested against AZD2563 included: Staphylococcus aureus ATCC 25923 and ATCC 29213, Enterococcus faecalis ATCC 29212 and Streptococcus pneumoniae ATCC 49619. In the eight-center QC trial, 320 and 480 results were generated for MIC and disk diffusion tests, respectively. The selected 7-mm ranges for the two disk diffusion QC organisms contained > or = 98.5% of reported results. Proposed 3 or 4 log(2) dilution MIC ranges contained all reported participant results. AZD2563 disk diffusion zone diameters were generally 2-3 mm smaller than the corresponding linezolid zone diameters. In conclusion, QC ranges for AZD2563 MIC and disk diffusion methods were established and appear acceptable in preparation for the clinical trials use.
AZD2563是一种新型长效恶唑烷酮，对革兰氏阳性菌具有强大的抗菌活性。本报告总结了AZD2563的肉汤微量稀释MIC和纸片扩散质量控制（QC）研究结果。所有测试或方法（M2 - A7和M7 - A5）以及QC研究设计（M23 - A2）均为美国国家临床实验室标准委员会发布的内容。针对AZD2563测试的QC菌株包括：金黄色葡萄球菌ATCC 25923和ATCC 29213、粪肠球菌ATCC 29212以及肺炎链球菌ATCC 49619。在八中心QC试验中，MIC和纸片扩散试验分别产生了320个和480个结果。两种纸片扩散QC菌株选定的7毫米范围包含≥98.5%的报告结果。提议的3或4个log(2)稀释MIC范围包含了所有报告的参与者结果。AZD2563纸片扩散区直径通常比相应的利奈唑胺区直径小2 - 3毫米。总之，已确定AZD2563 MIC和纸片扩散方法的QC范围，且在准备用于临床试验时似乎是可接受的。
