Monteville Marshall R, Yoon Julie E, Konkel Michael E
School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4233, USA.
Microbiology (Reading). 2003 Jan;149(Pt 1):153-65. doi: 10.1099/mic.0.25820-0.
The binding of Campylobacter jejuni to fibronectin (Fn), a component of the extracellular matrix, is mediated by a 37 kDa outer-membrane protein termed CadF for Campylobacter adhesion to fibronectin. The specificity of C. jejuni binding to Fn, via CadF, was demonstrated using antibodies reactive against Fn and CadF. More specifically, the anti-CadF antibody reduced the binding of two C. jejuni clinical isolates to immobilized Fn by greater than 50 %. Furthermore, a C. jejuni wild-type isolate, in contrast to the isogenic CadF mutant, was found to compete with another C. jejuni wild-type isolate for host cell receptors. Given the relationship between the pericellular Fn matrix and the cytoskeleton, the involvement of host cell cytoskeletal components in C. jejuni internalization was also examined. Cytochalasin D and mycalolide B microfilament depolymerizing agents resulted in a significant reduction in C. jejuni invasion. Studies targeting paxillin, a focal adhesion signalling molecule, identified an increased level of tyrosine phosphorylation upon C. jejuni infection of INT 407 cells. Collectively, these data suggest CadF promotes the binding of C. jejuni to Fn, which in turn stimulates a signal transduction pathway involving paxillin.
空肠弯曲菌与细胞外基质成分纤连蛋白（Fn）的结合由一种37 kDa的外膜蛋白介导，该蛋白称为CadF，负责空肠弯曲菌与纤连蛋白的黏附。通过使用针对Fn和CadF的抗体，证实了空肠弯曲菌通过CadF与Fn结合的特异性。更具体地说，抗CadF抗体使两种空肠弯曲菌临床分离株与固定化Fn的结合减少了50%以上。此外，与同基因CadF突变体相比，发现一株空肠弯曲菌野生型分离株能与另一株空肠弯曲菌野生型分离株竞争宿主细胞受体。鉴于细胞周围Fn基质与细胞骨架之间的关系，还研究了宿主细胞细胞骨架成分在空肠弯曲菌内化过程中的作用。细胞松弛素D和密钙霉素B等微丝解聚剂导致空肠弯曲菌入侵显著减少。针对桩蛋白（一种粘着斑信号分子）的研究发现，空肠弯曲菌感染INT 407细胞后酪氨酸磷酸化水平升高。总体而言，这些数据表明CadF促进空肠弯曲菌与Fn的结合，进而刺激涉及桩蛋白的信号转导途径。
