犬瘟热病毒血凝素（H）蛋白特异性细胞毒性T淋巴细胞在犬体内的活性

Cytotoxic T-lymphocyte activity specific for hemagglutinin (H) protein of canine distemper virus in dogs.

作者信息

Hirama Kyoko, Togashi Ken-ichi, Wakasa Chiaki, Yoneda Misako, Nishi Toshiya, Endo Yasuyuki, Miura Ryuichi, Tsukiyama-Kohara Kyoko, Kai Chieko

机构信息

Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Minato-ku, Japan.

出版信息

J Vet Med Sci. 2003 Jan;65(1):109-12. doi: 10.1292/jvms.65.109.

DOI:10.1292/jvms.65.109

PMID:12576714

Abstract

Cytotoxic T-lymphocyte (CTL) responses to hemagglutinin (H) protein of canine distemper virus (CDV) were evaluated in dogs using the replication-deficient adenovirus protein expression system. Skin fibroblasts were isolated from two dogs and were infected with recombinant adenovirus bearing the CDV-H gene (Ade-CDVH). CTL assay was performed using fibroblasts expressing CDV-H protein as target cells and peripheral blood lymphocytes (PBL) collected from the same dogs one week after immunization of CDV as effector cells. Specific cytotoxic activity was observed against autologous but not heterologous fibroblasts expressing CDV-H protein. These results indicate that the CTL epitope(s) were localized in the H protein.

摘要

使用复制缺陷型腺病毒蛋白表达系统在犬中评估了针对犬瘟热病毒（CDV）血凝素（H）蛋白的细胞毒性T淋巴细胞（CTL）反应。从两只犬分离皮肤成纤维细胞，并感染携带CDV-H基因的重组腺病毒（Ade-CDVH）。以表达CDV-H蛋白的成纤维细胞作为靶细胞，以在接种CDV一周后从同一只犬收集的外周血淋巴细胞（PBL）作为效应细胞进行CTL检测。观察到针对表达CDV-H蛋白的自体成纤维细胞而非异体成纤维细胞的特异性细胞毒性活性。这些结果表明CTL表位定位于H蛋白中。