犬瘟热病毒H蛋白的抗原差异

Antigenic differences in the H proteins of canine distemper viruses.

作者信息

Iwatsuki K, Tokiyoshi S, Hirayama N, Nakamura K, Ohashi K, Wakasa C, Mikami T, Kai C

机构信息

Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, Japan.

出版信息

Vet Microbiol. 2000 Feb;71(3-4):281-6. doi: 10.1016/s0378-1135(99)00172-8.

DOI:10.1016/s0378-1135(99)00172-8

PMID:10703710

Abstract

Antigenic properties between new Japanese field isolates and vaccine strains of canine distemper virus (CDV) have been compared using four monoclonal antibodies (MAbs) (JD-5, JD-7, JD-11 and d-7) against the hemagglutinin (H) proteins of CDV. JD-5, JD-7 and JD-11 are newly established antibodies. Three MAbs, namely d-7, JD-5 and JD-11, reacted similarly to all the CDV strains examined. However, JD-7 reacted much more strongly with the vaccine strains and an old field isolate than the recent field isolates in immunofluorescence, radio immunoprecipitation and virus neutralization assays. These results indicate that an antigenic region in the H protein, concerned with neutralization and recognized by JD-7, has been altered in the recent field isolates.

摘要

利用四种针对犬瘟热病毒（CDV）血凝素（H）蛋白的单克隆抗体（MAbs）（JD-5、JD-7、JD-11和d-7），比较了日本新的野外分离株与犬瘟热病毒疫苗株之间的抗原特性。JD-5、JD-7和JD-11是新建立的抗体。三种单克隆抗体，即d-7、JD-5和JD-11，对所有检测的CDV毒株反应相似。然而，在免疫荧光、放射免疫沉淀和病毒中和试验中，JD-7与疫苗株和一株旧的野外分离株的反应比与近期野外分离株的反应强烈得多。这些结果表明，H蛋白中与中和作用相关且能被JD-7识别的一个抗原区域在近期野外分离株中发生了改变。