Marotta Ester, Lapolla Annunziata, Fedele Domenico, Senesi Antonella, Reitano Rachele, Witt Matthias, Seraglia Roberta, Traldi Pietro
CNR, Istituto di Scienze e Tecnologie Molecolari, Corso Stati Uniti 4, 35100 Padova, Italy.
J Mass Spectrom. 2003 Feb;38(2):196-205. doi: 10.1002/jms.431.
The Maillard reaction occurring between sugars and amino groups is important in living systems. When amino groups belonging to protein chains are involved, the Maillard reaction has been invoked as responsible for protein cross-linking and the production of 'toxic' compounds. The reaction leads to the production of a heterogeneous group of substances, usually called advanced glycation end products (AGEs). Classical analytical approaches, such as spectroscopic (ultraviolet, fluorescence) and mass spectrometric (matrix-assisted laser desorption/ionization, liquid chromatography/electrospray ionization mass spectrometry) methods, have shown that the digestion mixture is highly complex. However, there are clear differences between the digestion mixtures of glycated and unglycated human serum albumin (HSA). In the former case, possible glycated peptides belonging to the AGE peptide class may be identified. Tandem mass spectrometric experiments on selected species seemed to be promising as regards structural information, but it was thought of interest to undertake the present investigation, based on liquid chromatography/electrospray ionization Fourier transform mass spectrometry, in order to obtain definitive results on their elemental composition. Using this approach, about 20 glycated peptides were detected and their possible structures were postulated by examining the known sequence of HSA.
糖与氨基之间发生的美拉德反应在生命系统中很重要。当涉及到蛋白质链中的氨基时，美拉德反应被认为是蛋白质交联和“有毒”化合物产生的原因。该反应会产生一组异质物质，通常称为晚期糖基化终产物（AGEs）。经典的分析方法，如光谱法（紫外、荧光）和质谱法（基质辅助激光解吸/电离、液相色谱/电喷雾电离质谱），表明消化混合物非常复杂。然而，糖化和未糖化的人血清白蛋白（HSA）的消化混合物之间存在明显差异。在前一种情况下，可以鉴定出属于AGE肽类的可能糖化肽。对选定物种进行的串联质谱实验在结构信息方面似乎很有前景，但基于液相色谱/电喷雾电离傅里叶变换质谱进行本研究被认为很有意义，以便获得关于其元素组成的确切结果。使用这种方法，检测到了约20种糖化肽，并通过检查HSA的已知序列推测了它们可能的结构。
