Gao Li-hong, Liu Hong-lai, Chai Shui-hong, Cai Yi-feng, Liu Li-li, Wu Yu-tian
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Yao Xue Xue Bao. 2002 May;37(5):355-8.
To establish a suitable condition for extraction of phenylalanine (Phe), 5-hydroxytryptophan (5-OH-Trp) and four diastereomeric salts, (1R,2S)-ephedrine- (2S,3S)-tartaric acid, (1R,2S)-ephedrine-(2R,3R)-tartaric acid, (1S,2S)-pseudoephedrine-(2S,3S)-tartaric acid, (1S,2S)-pseudoephedrine- (2R,3R)-tartaric acid in supercritical fluid extraction and to assess the solubilities of Phe, 5-OH-Trp and the four diastereomeric salts in CO2.
Single-pass method and HPCE.
The solubilities of Phe, 5-OH-Trp and the four diastereomeric salts in CO2 were determined over temperature and pressure ranges of 25-50 degrees C and 6.32-34.03 MPa respectively. The experimental results showed that the solubilities of Phe, 5-OH-Trp and the four diastereomeric salts do not increase with density of CO2. There existed a maximum in the critical region of CO2.
The dramatically high solubilities in the pressure of 6.32-7.78 MPa show a critical behavior, which can be explained by critical characteristic through thermodynamics analysis. The results suggest that the separation of Phe, 5-OH-Trp and the four diastereomeric salts is more efficient in critical region of CO2.
建立在超临界流体萃取中提取苯丙氨酸（Phe）、5-羟色氨酸（5-OH-Trp）以及四种非对映体盐，即（1R,2S）-麻黄碱-（2S,3S）-酒石酸、（1R,2S）-麻黄碱-（2R,3R）-酒石酸、（1S,2S）-伪麻黄碱-（2S,3S）-酒石酸、（1S,2S）-伪麻黄碱-（2R,3R）-酒石酸的适宜条件，并评估Phe、5-OH-Trp和四种非对映体盐在二氧化碳中的溶解度。
单程法和高效毛细管电泳法。
分别在25 - 50℃和6.32 - 34.03 MPa的温度和压力范围内测定了Phe、5-OH-Trp和四种非对映体盐在二氧化碳中的溶解度。实验结果表明，Phe、5-OH-Trp和四种非对映体盐的溶解度并不随二氧化碳密度的增加而增大。在二氧化碳的临界区域存在一个最大值。
在6.32 - 7.78 MPa压力下显著高的溶解度表现出临界行为，通过热力学分析可由临界特性来解释。结果表明，在二氧化碳的临界区域分离Phe、5-OH-Trp和四种非对映体盐更有效。
