[云芝子实体多糖化学结构的研究]

[Studies on chemical structure of polysaccharide from fruit body of Coriolus versicolor].

作者信息

Zhang J S, Han W W, Pan Y J

机构信息

Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

出版信息

Yao Xue Xue Bao. 2001 Sep;36(9):664-7.

PMID:12580103

Abstract

AIM

To study the structure of polysaccharides from Coriolus versicolor.

METHODS

By chromatography a homogeneous polysaccharide B-1-3 was obtained. The structure of B-1-3 was elucidated using the method of total acid hydrolysis, methylation, and 1D and 2D NMR.

RESULTS

B-1-3 was found to be mainly composed of Glc by total acid hydrolysis. Its molecular weight was estimated to be 3.16 x 10(5) by HPLC method. Methylation analysis and 1H and 13CNMR indicated the main chain of B-1-3 consisted of beta-D-1,4-Glc and beta-D-1,3-Glc, and branch chains were situated, beta-D-1,3,6-Glc and beta-D-1,4,6-Glc. 1H and 13CNMR also showed that beta-D-Glc-1-->3-beta-D-Glc-beta-D-Glc-1-->4-beta-D-Glc and beta-D-Glc-1-->4-beta-D-Glc-beta-D-Glc-1-->4-beta-D-Glc fragments existed in B-1-3.

CONCLUSION

B-1-3 is a glucan which main chain was composed of beta-D-1,4-Glc and beta-D-1,3-Glc beta-D-1,6-Glc.

摘要

目的

研究云芝多糖的结构。

方法

通过色谱法得到一种均一多糖B - 1 - 3。采用全酸水解、甲基化以及一维和二维核磁共振方法阐明B - 1 - 3的结构。

结果

全酸水解表明B - 1 - 3主要由葡萄糖组成。通过高效液相色谱法估计其分子量为3.16×10⁵。甲基化分析以及¹H和¹³C核磁共振表明B - 1 - 3的主链由β - D - 1,4 - 葡萄糖和β - D - 1,3 - 葡萄糖组成，支链位于β - D - 1,3,6 - 葡萄糖和β - D - 1,4,6 - 葡萄糖处。¹H和¹³C核磁共振还表明B - 1 - 3中存在β - D - 葡萄糖 - 1→3 - β - D - 葡萄糖 - β - D - 葡萄糖 - 1→4 - β - D - 葡萄糖和β - D - 葡萄糖 - 1→4 - β - D - 葡萄糖 - β - D - 葡萄糖 - 1→4 - β - D - 葡萄糖片段。