Xu De-Sheng, Feng Yi, Lin Xiao, Deng Hai-Lin, Fang Ji-Nian, Dong Qun
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Yao Xue Xue Bao. 2005 Jul;40(7):636-9.
To separate and purify the anti-myocardial ischemic polysaccharide fraction with a homogenous molecular weight from Ophiopogon japonicus, then study the chemical structure of the parts.
Crude polysaccharides were prepared by extracting the tube root fraction of Ophiopogon japonicus with water, then precipitation with ethanol. From the crude polysaccharides, the polysaccharide of MDG-1 was separated and purified using ultrafiltration, DEAE Sepharose FF and Sephadex G-25 column chromatography. Its structure was studied by complete hydrolysis, periodate oxidation, Smith degradation, methylation analysis, 1H NMR and 13C NMR analysis etc.
MDG-1 was a water-soluble beta-D-fructosan, containing a backbone composed of Fruf (2 --> 1), and a branch of Fruf (2 --> 6) Fruf (2 --> per average 2. 8 of main chain residues. Mn, Mw and Mp of MDG-1 were 3 400, 4 800 and 5 000, respectively. MDG-1 contains trace of Glc, which maybe connect to its reducing terminal. Molar ratio of Fru and Glc is approximately 35: 1.
从麦冬中分离纯化出具有均一分子量的抗心肌缺血多糖组分，并研究其化学结构。
用水提取麦冬块根部位得到粗多糖，再用乙醇沉淀。从粗多糖中，通过超滤、DEAE 琼脂糖 FF 和葡聚糖凝胶 G - 25 柱色谱法分离纯化出 MDG - 1 多糖。通过完全水解、高碘酸氧化、史密斯降解、甲基化分析、1H NMR 和 13C NMR 分析等方法研究其结构。
MDG - 1 是一种水溶性的β - D - 果聚糖，其主链由 Fruf（2→1）组成，且有一个 Fruf（2→6）Fruf（2→ 平均每 2.8 个主链残基）的分支。MDG - 1 的 Mn、Mw 和 Mp 分别为 3400、4800 和 5000。MDG - 1 含有痕量的 Glc，可能连接在其还原端。Fru 和 Glc 的摩尔比约为 35∶1。
