Norikane J, Goto E, Kurata K, Takakura T
Dynamac Corp., Kennedy Space Center, Florida 32899, USA.
Adv Space Res. 2003;31(1):245-8. doi: 10.1016/s0273-1177(02)00746-9.
The measurement of plant chlorophyll fluorescence has been used for many years as a method to monitor a plant's health status. These types of methods have been mostly relegated to the laboratory. The newly developed Relative Referencing Method allows for the measurement of chlorophyll fluorescence under artificial lighting conditions. The fluorescence signal can be determined by first taking a reference signal measurement, then a second measurement with an additional fluorescence excitation source. The first signal can then be subtracted from the second and the plant's chlorophyll fluorescence due to the second lighting source can be determined. With this simple approach, a photosynthesizing plant can be monitored to detect signs of water stress. Using this approach experiments on tomato plants have shown that it was possible to detect water stress, while the plants were continuously illuminated by fluorescent lamps. This method is a promising tool for the remote monitoring of crops grown in a CELSS-type application.
植物叶绿素荧光测量作为监测植物健康状况的一种方法已被使用多年。这类方法大多局限于实验室。新开发的相对参考法允许在人工光照条件下测量叶绿素荧光。荧光信号可通过先测量参考信号，然后使用额外的荧光激发源进行第二次测量来确定。然后从第二次测量值中减去第一次测量值，就可以确定由于第二个光源产生的植物叶绿素荧光。通过这种简单的方法，可以监测进行光合作用的植物以检测水分胁迫迹象。利用这种方法对番茄植株进行的实验表明，在荧光灯持续照射植株的情况下，有可能检测到水分胁迫。该方法是用于远程监测在CELSS型应用中种植的作物的一种很有前景的工具。
