Sengler C, Haider A, Sommerfeld C, Lau S, Baldini M, Martinez F, Wahn U, Nickel R
Department of Pediatric Pneumology and Immunology, Charité- Humboldt University, Berlin, Germany.
Clin Exp Allergy. 2003 Feb;33(2):166-9. doi: 10.1046/j.1365-2222.2003.01549.x.
Multiple genetic studies have shown linkage of atopy-related phenotypes to chromosome 5q31. In this region several candidate genes for atopy are localized such as the Th2 cytokines IL-4, IL-5 and IL-13, but also CD14, a receptor for LPS. Recently, a functional CD14 promoter polymorphism was related to total and specific IgE responsiveness.
The aim of our study was to evaluate the role of this single nucleotide polymorphism (SNP) in a large German birth cohort.
Atopy-related phenotypes were longitudinally carefully evaluated in over 800 children from birth to the age of 10 years. Yearly visits included standardized interviews, physical examinations and determination of total and specific IgE antibodies. Pulmonary function tests and histamine provocations were performed at the age of seven. Eight-hundred and seventy-two children of the Multicenter Allergy Study (MAS) cohort were genotyped using melting curve and restriction digest analyses.
CD14-159 allele frequencies were consistent with previous reports, however, no association of the SNP with asthma, atopic dermatitis, allergic rhinitis, total or specific IgE levels could be observed.
The CD14-159 SNP might not play a major role in the development of atopy in German children.
多项基因研究表明，特应性相关表型与5号染色体q31区域存在连锁关系。在该区域定位了几个特应性候选基因，如Th2细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）和白细胞介素-13（IL-13），还有脂多糖受体CD14。最近，一种功能性CD14启动子多态性与总IgE及特异性IgE反应性相关。
我们研究的目的是评估这种单核苷酸多态性（SNP）在一个大型德国出生队列中的作用。
对800多名儿童从出生到10岁进行了纵向的特应性相关表型仔细评估。每年的随访包括标准化访谈、体格检查以及总IgE和特异性IgE抗体的测定。在7岁时进行肺功能测试和组胺激发试验。使用熔解曲线和限制性酶切分析对多中心过敏研究（MAS）队列中的872名儿童进行基因分型。
CD14 - 159等位基因频率与先前报道一致，然而，未观察到该SNP与哮喘、特应性皮炎、过敏性鼻炎、总IgE或特异性IgE水平之间存在关联。
CD14 - 159 SNP可能在德国儿童特应性疾病的发生中不起主要作用。
