Faculty of Medicine, Center for Human Molecular Genetics and Pharmacogenomics, University of Maribor, Maribor, Slovenia.
Respir Med. 2011 Oct;105 Suppl 1:S54-9. doi: 10.1016/S0954-6111(11)70012-9.
Though the associations of CD14 with asthma have already been studied, the results of different independent studies are in conflict, mostly due to differences in the pathogenesis of varying asthma sub-phenotypes. The aim of our study was to perform an association analysis of promoter single nucleotide polymorphism (SNP) -159C/T (rs2569190) in the CD14 gene for Slovenian children with asthma.
We analyzed SNP -159C/T in a group of all asthmatics, and separately in a group of atopic and non-atopic asthmatics. We also analyzed the influence of SNP -159C/T on clinical parameters and the response to therapy with inhaled corticosteroids. We have genotyped 247 children with asthma and a median age of 11 years (interquartile range, 5 years), and 158 healthy controls with a median age of 13 years (interquartile range, 5 years). We performed genotyping using a polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis.
We found that SNP -159C/T in CD14 is not associated with asthma in Slovenian children. However, non-atopic asthmatics with CT or TT genotypes have a lower FEV1/FVC ratio as a measure of bronchial obstruction (87.4%, compared to 91.8% in patients with the CC genotype, p = 0.017). Non-atopic asthmatics with CC or CT genotypes also have increased bronchial hyperreactivity measured by PC20 of methacholine (0.41 mg/ml, compared to 1.50 mg/ml in patients with a TT genotype, p = 0.018).
Our results suggest that CD14 is associated with asthma severity in Slovenian children with non-atopic asthma.
虽然 CD14 与哮喘的关联已经得到研究，但不同独立研究的结果存在冲突，主要是由于不同哮喘亚表型的发病机制存在差异。我们的研究目的是对斯洛文尼亚哮喘儿童 CD14 基因启动子单核苷酸多态性（SNP）-159C/T（rs2569190）进行关联分析。
我们分析了哮喘患者组、特应性哮喘组和非特应性哮喘组中 SNP-159C/T 的情况。我们还分析了 SNP-159C/T 对临床参数和吸入性皮质类固醇治疗反应的影响。我们对 247 名哮喘儿童（中位年龄 11 岁，四分位间距 5 岁）和 158 名健康对照者（中位年龄 13 岁，四分位间距 5 岁）进行了 SNP-159C/T 基因分型。我们使用聚合酶链反应（PCR）后限制性片段长度多态性（RFLP）分析进行基因分型。
我们发现，CD14 中的 SNP-159C/T 与斯洛文尼亚儿童哮喘无关。然而，CT 或 TT 基因型的非特应性哮喘患者的 FEV1/FVC 比值较低，表明存在支气管阻塞（87.4%，而 CC 基因型患者为 91.8%，p=0.017）。CC 或 CT 基因型的非特应性哮喘患者的气道高反应性也较高，以乙酰甲胆碱 PC20 衡量（0.41mg/ml，而 TT 基因型患者为 1.50mg/ml，p=0.018）。
我们的结果表明，CD14 与斯洛文尼亚非特应性哮喘儿童哮喘严重程度有关。
