Georgi L., Wang Y., Yvergniaux D., Ormsbee T., Iñigo M., Reighard G., Abbott G.
Department of Genetics and Biochemistry, Clemson University, Clemson SC 29634, USA,
Theor Appl Genet. 2002 Dec;105(8):1151-1158. doi: 10.1007/s00122-002-0967-4. Epub 2002 Sep 4.
The Rosaceae contains many economically important crop species, but their genomes are not well characterized, and comparative genetic mapping lags well behind that of other families. To facilitate genome comparisons and gene discovery in the Rosaceae, we have begun the development of genomic resources for peach as the model genome for this family. First, we developed a simplified, cost-effective method for constructing BAC libraries, particularly appropriate for plant species of relatively minor economic importance. Second, we used the library to investigate the abundance and local distribution of simple sequence repeats (SSRs) in peach. Our results indicate that microsatellite loci are locally much more highly abundant than previously estimated, and BAC sequencing results suggest that microsatellite repeats are not randomly distributed within gene-containing regions of the peach genome. This makes it relatively easy to identify SSRs in peach by hybridization to BAC clones, and even by random sequencing of BAC clones, not known a priori to contain SSRs.
蔷薇科包含许多具有重要经济价值的作物品种，但其基因组尚未得到很好的表征，而且比较遗传图谱绘制远远落后于其他科。为了促进蔷薇科的基因组比较和基因发现，我们已开始开发桃的基因组资源，将其作为该科的模式基因组。首先，我们开发了一种简化的、经济高效的BAC文库构建方法，特别适用于经济重要性相对较小的植物物种。其次，我们利用该文库研究了桃中简单序列重复（SSR）的丰度和局部分布。我们的结果表明，微卫星位点在局部的丰度比先前估计的要高得多，并且BAC测序结果表明微卫星重复在桃基因组的含基因区域内并非随机分布。这使得通过与BAC克隆杂交，甚至通过对事先不知道含有SSR的BAC克隆进行随机测序，在桃中鉴定SSR相对容易。
