Tekeoglu M., Rajesh N., Muehlbauer J.
USDA-ARS, Department of Crop and Soil Sciences, 303 Johnson Hall, Washington State University, Pullman, WA 99164-6434, USA.
Theor Appl Genet. 2002 Nov;105(6-7):847-854. doi: 10.1007/s00122-002-0993-2. Epub 2002 Jul 2.
Fifty sequence-tagged microsatellite site (STMS) markers and a resistant gene-analog (RGA) locus were integrated into a chickpea ( Cicer arietinum L., 2n = 2 x = 16 chromosomes) genetic map that was previously constructed using 142 F(6)-derived recombinant inbred lines (RILs) from a cross of C. arietinum x Cicer reticulatum Lad. The map covers 1,174.5 cM with an average distance of 7.0 cM between markers in nine linkage groups (LGs). Nine markers including the RGA showed distorted segregation ( P < 0.05). The majority of the newly integrated markers were mapped to marker-dense regions of the LGs. Six co-dominant STMS markers were integrated into two previously reported major quantitative trait loci (QTLs) conferring resistance to Ascochyta blight caused by Ascochyta rabiei (Pass.) Labr. Using common STMS markers as anchors, three maps developed from different mapping populations were joined, and genes for resistance to Ascochyta blight, Fusarium wilt (caused by Fusarium oxysporum Schlechtend.: Fr. f. sp. ciceris), and for agronomically important traits were located on the combined linkage map. The integration of co-dominant STMS markers improves the map of chickpea and makes it possible to consider additional fine mapping of the genome and also map-based cloning of important disease resistance genes.
五十个序列标签微卫星位点（STMS）标记和一个抗病基因类似物（RGA）位点被整合到鹰嘴豆（Cicer arietinum L.，2n = 2x = 16条染色体）遗传图谱中，该图谱先前是利用来自C. arietinum与Cicer reticulatum Lad杂交的142个F(6)衍生重组自交系（RILs）构建的。该图谱覆盖1174.5厘摩，九个连锁群（LGs）中标记间平均距离为7.0厘摩。包括RGA在内的九个标记表现出分离扭曲（P < 0.05）。大多数新整合的标记被定位到LGs的标记密集区域。六个共显性STMS标记被整合到两个先前报道的主要数量性状位点（QTLs）中，这些位点赋予对由Ascochyta rabiei（Pass.）Labr引起的褐斑病的抗性。以常见的STMS标记为锚定，将来自不同作图群体构建的三张图谱进行合并，并且将抗褐斑病、枯萎病（由Fusarium oxysporum Schlechtend.: Fr. f. sp. ciceris引起）的基因以及农艺重要性状的基因定位到合并后的连锁图谱上。共显性STMS标记的整合改进了鹰嘴豆图谱，使得对基因组进行额外的精细作图以及对重要抗病基因进行基于图谱的克隆成为可能。
