Pi Wenxia, Cai Baochang, Pan Yang, Qian Jihong, Jia Ying
Nanjing University of Traditional Chinese Medicine, Nanjing 210029.
Zhong Yao Cai. 2002 May;25(5):349-51.
To study the technological parameters of the enrichment purification of Cornus officinalis glycosides (COG) with macroporous resin and active carbon.
COG was isolated and purified by active carbon and macroporous resin HPD100. The enrichment of COG was evaluated by HPLC method.
The optimum process was that 10 ml extract of Fructus Corni was absorbed with columns of macroporous resin and active carbon(R25 mm x H300 mm) and the columns were washed with 600 ml distilled water, then the morroniside and loganin was eluted from the macroporous resin and active carbon with ethanol.
The elutive rate of morroniside and logann was about 85% by the macroporous resin, 96% by the active carbon. So this method enriching COG from Fructus Corni is practical.
