Dobigny G, Ozouf-Costaz C, Bonillo C, Volobouev V
Laboratoire Mammifères and Oiseaux, Muséum National d'Histoire Naturelle, Paris, France.
Cytogenet Genome Res. 2002;98(1):75-7. doi: 10.1159/000068541.
In spite of uncertainty about the biochemical processes involved, silver staining is a widely used technique for assessing the locations of active NORs in eukaryotic genomes in general, and in mammalian genomes in particular. However, following a previous study of hedgehog chromosomes, we present here a second example from two gerbil species (Rodentia, Muridae), which have several clear Ag-positive signals that do not correspond to 28S rDNA clusters. Although this pattern may be characteristic of particular genomes displaying unusual heterochromatic features, our study casts doubt upon the reliability and universality of Ag-staining for detecting active NORs.
尽管所涉及的生化过程尚不确定，但银染法是一种广泛应用的技术，通常用于评估真核生物基因组中活性核仁组织区（NORs）的位置，尤其是哺乳动物基因组。然而，在先前对刺猬染色体的研究之后，我们在此展示来自两个沙鼠物种（啮齿目，鼠科）的第二个例子，它们有几个清晰的银染阳性信号，这些信号并不对应于28S rDNA簇。尽管这种模式可能是显示异常异染色质特征的特定基因组所特有的，但我们的研究对银染法检测活性NORs的可靠性和普遍性提出了质疑。
