吸水链霉菌NRRL 3602中格尔德霉素生物合成基因簇的克隆与鉴定
Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602.
作者信息
Rascher Andreas, Hu Zhihao, Viswanathan Nina, Schirmer Andreas, Reid Ralph, Nierman William C, Lewis Matthew, Hutchinson C Richard
机构信息
Kosan Biosciences, 3832 Bay Center Place, 94545, Hayward, CA, USA.
出版信息
FEMS Microbiol Lett. 2003 Jan 28;218(2):223-30. doi: 10.1016/S0378-1097(02)01148-5.
We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.
我们展示了一种基于聚合酶链式反应（PCR）的方法的应用，通过该方法可快速检测微生物的基因组DNA中是否存在I型模块化聚酮合酶基因，以便通过序列分析和基因破坏来克隆和表征来自吸水链霉菌变株盖尔达霉素链霉菌NRRL 3602的格尔德霉素生产基因簇的主要部分。
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