Bordignon Nunes Fernanda, Meier Graziottin Cássio, Alves Filho José Carlos Farias, Lunardelli Adroaldo, Caberlon Eduardo, Peres Alessandra, Rodrigues De Oliveira Jarbas
Laboratório de Pesquisa em Biofísica, Departamento de Ciências Fisiológicas, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Av Ipiranga, 6681-Prédio 12C-Sala 263-CP 1429, Porto Alegre, RS, Brazil.
Int Immunopharmacol. 2003 Feb;3(2):267-72. doi: 10.1016/S1567-5769(02)00295-3.
Sepsis remains an important and life-threatening problem, and is the most common cause of death in the intensive care unit. One promising therapeutic candidate for protection against injury in sepsis is fructose-1,6-bisphosphate (FBP), a high-energy glycolytic pathway intermediate. The objective of the study was to establish a role for FBP on the immune system, especially in lymphocyte proliferation. Peripheral blood mononuclear cells (PBMCs) were isolated from the blood of healthy humans by gradient centrifugation. T-lymphocytes were stimulated for 96 h with phytohemagglutinin (PHA) and varying concentration of FBP. Fructose-1,6-bisphosphate at concentrations between 1.2 and 10 mM decreased proliferation of T-lymphocytes and reduced the viability only at concentrations 5.0 and 10 mM. The levels of soluble IL-2 receptor were reduced at FBP concentrations between 1.2 and 10 mM. In conclusion, this study demonstrates that FBP has important effect on immunomodulatory and this result can be correlated with the protection against injury in sepsis.
脓毒症仍然是一个重要且危及生命的问题，并且是重症监护病房中最常见的死亡原因。一种有望用于预防脓毒症损伤的治疗候选物是1,6-二磷酸果糖（FBP），它是高能糖酵解途径的中间产物。该研究的目的是确定FBP在免疫系统中的作用，尤其是在淋巴细胞增殖方面的作用。通过梯度离心从健康人的血液中分离出外周血单核细胞（PBMC）。用植物血凝素（PHA）和不同浓度的FBP刺激T淋巴细胞96小时。浓度在1.2至10 mM之间的1,6-二磷酸果糖会降低T淋巴细胞的增殖，并且仅在浓度为5.0和10 mM时才会降低细胞活力。在FBP浓度为1.2至10 mM之间时，可溶性白细胞介素-2受体水平降低。总之，本研究表明FBP具有重要的免疫调节作用，并且该结果可能与预防脓毒症损伤相关。
