迈向一种用于测定鱼类肌肉氧化还原底物的有效采样技术。
Towards a valid technique of sampling fish muscle to determine redox substrates.
作者信息
Börjeson H, Fellenius E
出版信息
Acta Physiol Scand. 1976 Feb;96(2):202-6. doi: 10.1111/j.1748-1716.1976.tb10189.x.
The redox substrates--lactate, malate, alpha-glycerophosphate, dihydroxy acetonephosphate and pyruvate--have been determined in liver and muscle tissue from young salmon. The redox quotients have also been calculated. The freeze clamping technique was used and reliable samples of fish muscle for the determination of lactate were obtained with the aid of a pair of pliers with a gear mechanism. It was established that the lactate content in the body muscle is in close agreement with that in the blood from rested salmon parr. The concentrations of redox substrates are in good agreement with those found in mammalian tissue. The determination of the content of glycogen in fish muscle is discussed in the light of the results obtained in this study.
已测定了幼鲑肝脏和肌肉组织中的氧化还原底物——乳酸、苹果酸、α-甘油磷酸、磷酸二羟丙酮和丙酮酸。还计算了氧化还原商。采用了冷冻钳夹技术，并借助带有齿轮机构的钳子获得了用于测定乳酸的可靠鱼肌肉样本。已确定体肌中的乳酸含量与静息鲑鱼苗血液中的乳酸含量密切一致。氧化还原底物的浓度与在哺乳动物组织中发现的浓度高度一致。根据本研究获得的结果，讨论了鱼肌肉中糖原含量的测定。
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