Sahni Rohini, Gupta Shrish C
Department of Botany, University of Delhi, Delhi 110007, India.
Indian J Exp Biol. 2002 Sep;40(9):1050-5.
Multiple shoots were initiated after 20 days in stem nodes excised from in vitro grown seedlings of Acacia catechu, on Murashige and Skoog's medium adjuvanted with 1 to 100 microM of N6-benzyladenine (BA). Explants were subcultured on the same medium augmented with 1.5 g l(-1) of polyvinylpyrrolidone (PVP) after 30 days. In the second subculture, after 30 days, the explants were transferred to a medium lacking PVP, but containing 10 microM of BA, where nine or ten shoots differentiated per explant within next 30 days. If individual shoots along with some callus were subcultured on BA (10 microM), nearly 15 shoots per explant regenerated in 90 days. Thus, the average number of shoots obtained from each node was 142 after 180 days. Since a seedling develops four nodes after 20 days, theoretically an average of 568 shoots can be obtained from a single seed. If shoots were individually subcultured on 1/2-strength MS medium with 14.7 microM of indole-3-butyric acid (IBA), roots developed in 20 days. Addition of 40 mg l(-1) of glutamic acid to the rooting medium prevented leaf senescence. These plantlets thrived well in garden soil, sand and silica (1:1:1).
从儿茶离体培养的幼苗切取的茎节在添加了1至100微摩尔N6-苄基腺嘌呤（BA）的Murashige和Skoog培养基上培养20天后开始产生多个芽。30天后，将外植体转移到添加了1.5克/升聚乙烯吡咯烷酮（PVP）的相同培养基上进行继代培养。在第二次继代培养30天后，将外植体转移到不含PVP但含有10微摩尔BA的培养基上，在接下来的30天内每个外植体分化出9或10个芽。如果将单个芽连同一些愈伤组织在BA（10微摩尔）上进行继代培养，每个外植体在90天内可再生近15个芽。因此，180天后每个节点获得的芽的平均数量为142个。由于幼苗在20天后长出4个节点，理论上从一粒种子平均可获得568个芽。如果将芽单独在含有14.7微摩尔吲哚-3-丁酸（IBA）的1/2强度MS培养基上进行继代培养，20天内可生根。在生根培养基中添加40毫克/升谷氨酸可防止叶片衰老。这些小植株在园土、沙子和硅石（1:1:1）中生长良好。
