Maity S K, De K K, Kundu A K
Department of Botany, Barasat Government College, Parganas, India.
Indian J Exp Biol. 2001 Nov;39(11):1188-90.
Axillary bud explants were induced to form shoots on Murashige and Skoog's (MS)' basal medium. Best yield (9 shoots per explant) was obtained when the medium was supplemented with adenine sulphate (40 mg/L) and 6-benzylaminopurine (2.25 mg/L). The shoots were rooted on half strength MS basal medium supplemented with indole butyric acid (0.5 mg/L) and having thiamin-HCl (800 mg/L). Regenerated plantlets were successfully acclimatized. This is the first report of micropropagation in the genus Mussaenda without callus intervention.
将腋芽外植体接种于Murashige和Skoog（MS）基本培养基上诱导形成芽。当培养基添加硫酸腺嘌呤（40毫克/升）和6-苄基腺嘌呤（2.25毫克/升）时，获得了最佳产量（每个外植体9个芽）。将芽接种于添加了吲哚丁酸（0.5毫克/升）和盐酸硫胺素（800毫克/升）的1/2强度MS基本培养基上生根。再生植株成功驯化。这是关于玉叶金花属植物在无愈伤组织干预下进行微繁殖的首次报道。
