Chuchalin A G, Novoselov V I, Shifrina O N, Soodaeva S K, Yanin V A, Barishnikova L M
Pulmonology Research Institute, Moscow, Russia.
Respir Med. 2003 Feb;97(2):147-51. doi: 10.1053/rmed.2003.1429.
Peroxiredoxins (Prxs) constitute a novel family of antioxidant proteins, which specifically prevent enzymes from metal-catalyzed oxidation. The localization of a member of the mono-cystein subfamily of Prxs, Prx VI in human respiratory system and its antioxidant properties were investigated. By immunoblotting, the Prx VI was found to be present in human respiratory epithelium. Immunostaining with rabbit polyclonal antibody raised against the Prx VI revealed that the said protein was present in apical areas and mucus of all respiratory airways from trachea to bronchioles. Immunodepletion of the Prx VI profoundly decreased the antioxidant activity of the respiratory epithelium extract.
过氧化物酶（Prxs）构成了一个新的抗氧化蛋白家族，它能特异性地防止酶发生金属催化氧化。研究了Prxs单半胱氨酸亚家族成员Prx VI在人类呼吸系统中的定位及其抗氧化特性。通过免疫印迹法发现Prx VI存在于人类呼吸道上皮中。用针对Prx VI产生的兔多克隆抗体进行免疫染色显示，该蛋白存在于从气管到细支气管的所有呼吸道的顶端区域和黏液中。Prx VI的免疫去除显著降低了呼吸道上皮提取物的抗氧化活性。
