Novoselov S V, Peshenko I V, Popov V I, Novoselov V I, Bystrova M F, Evdokimov V J, Kamzalov S S, Merkulova M I, Shuvaeva T M, Lipkin V M, Fesenko E E
Institute of Cell Biophysics, Russian Academy of Sciences, Moscow region, Russia.
Cell Tissue Res. 1999 Dec;298(3):471-80. doi: 10.1007/s004419900115.
Peroxiredoxins are a novel family of antioxidant proteins that specifically prevent enzymes from metal-catalyzed oxidation. The localization of a member of the mono-cystein subfamily of peroxiredoxins, the 28-kDa protein, in different rat tissues and its antioxidant properties were investigated. By immunoblotting, the 28-kDa peroxiredoxin was found to be most highly concentrated in olfactory epithelium and present in all tissues tested (skin, lung, trachea, kidney, womb, and brain). Immunostaining with rabbit polyclonal antibody raised against the 28-kDa peroxiredoxin revealed the particularly high level of the 28-kDa peroxiredoxin immunoreactivity in air-contacting areas (apical regions and mucus of the olfactory and respiratory epithelium and skin epidermis), which are continually exposed to numerous air-borne reactive oxygen species. In the apical regions of the olfactory and respiratory epithelium, the 28-kDa-peroxiredoxin immunogold labeling outlined microvilli and cilia and was mainly located in sustentacular cells and in respiratory and goblet cells, as electron-microscopic analysis revealed. In skin epidermis, the 28-kDa peroxiredoxin immunoreactivity was confined to the granular layer and specifically concentrated in sebaceous glands of hair follicle. In situ hybridization with 33P-labeled antisense RNA probe revealed the expression of the 28-kDa peroxiredoxin mRNA in tissues with a high level of the 28-kDa peroxiredoxin immunoreactivity. Immunodepletion of the 28-kDa peroxiredoxin profoundly decreased the antioxidant activity of the olfactory tissue extract.
过氧化物酶是一类新型抗氧化蛋白家族，可特异性防止酶发生金属催化氧化。研究了过氧化物酶单半胱氨酸亚家族成员28 kDa蛋白在不同大鼠组织中的定位及其抗氧化特性。通过免疫印迹法发现，28 kDa过氧化物酶在嗅觉上皮中浓度最高，且在所检测的所有组织（皮肤、肺、气管、肾、子宫和脑）中均有表达。用针对28 kDa过氧化物酶的兔多克隆抗体进行免疫染色显示，在与空气接触的区域（嗅觉和呼吸道上皮以及皮肤表皮的顶端区域和黏液）中，28 kDa过氧化物酶免疫反应性特别高，这些区域持续暴露于大量空气传播的活性氧中。电子显微镜分析显示，在嗅觉和呼吸道上皮的顶端区域，28 kDa过氧化物酶免疫金标记勾勒出微绒毛和纤毛，主要位于支持细胞以及呼吸道和杯状细胞中。在皮肤表皮中，28 kDa过氧化物酶免疫反应性局限于颗粒层，并特别集中在毛囊的皮脂腺中。用33P标记的反义RNA探针进行原位杂交显示，在28 kDa过氧化物酶免疫反应性高的组织中存在28 kDa过氧化物酶mRNA的表达。28 kDa过氧化物酶的免疫耗竭显著降低了嗅觉组织提取物的抗氧化活性。
