Arai T, Nakamura M, Magori E, Fukuda H, Sako T
Department of Veterinary Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
Res Vet Sci. 2003 Apr;74(2):183-5. doi: 10.1016/s0034-5288(02)00177-7.
The activities of the enzymes involved in the malate-aspartate shuttle were measured in peripheral leucocytes of dogs with type 1 diabetes mellitus. In the diabetic dogs, fasting plasma glucose concentrations were twofold greater than control levels despite insulin injections and the activities of malate dehydrogenase (MDH), which plays a crucial role in the malate-aspartate shuttle, were decreased remarkably. The cytosolic ratio of MDH/lactate dehydrogenase (LDH) activity (M/L ratio) in leucocytes of the diabetic dogs was significantly lower than that of normal control dogs. The decrease of the M/L ratio appeared to reflect depression of energy metabolism in leucocytes of the diabetic dogs. The M/L ratio may be a useful parameter to evaluate metabolic conditions in diabetic dogs.
在1型糖尿病犬的外周白细胞中测量了参与苹果酸-天冬氨酸穿梭的酶的活性。在糖尿病犬中，尽管注射了胰岛素，但空腹血浆葡萄糖浓度仍比对照水平高两倍，而在苹果酸-天冬氨酸穿梭中起关键作用的苹果酸脱氢酶（MDH）的活性显著降低。糖尿病犬白细胞中MDH/乳酸脱氢酶（LDH）活性的胞质比率（M/L比率）明显低于正常对照犬。M/L比率的降低似乎反映了糖尿病犬白细胞能量代谢的抑制。M/L比率可能是评估糖尿病犬代谢状况的一个有用参数。
