Sanyal A, Gurumurthy C B, Venkataramanan R, Hemadri D, Tosh C
Project Directorate on Foot-and-Mouth Disease, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon, Nainital 263138, Uttaranchal, India.
Vet Microbiol. 2003 May 2;93(1):1-11. doi: 10.1016/s0378-1135(02)00425-x.
Foot-and-mouth disease virus (FMDV) serotype Asia1 field isolates (n = 100) were compared using a panel of 11 monoclonal antibodies (Mab) in sandwich ELISA. The majority (over 89%) of the isolates showed either homologous (76% and above reactivity) or reduced affinity (20-75% reactivity) for the Mabs 2A, 13, 40, 34 and 81, suggesting that these Mab binding epitopes are conserved, whereas a more variable reactivity was observed for the Mabs B3, 1A, 24, 72, 82 and 89. Polyclonal relationship ('r' value) of the field isolates in liquid phase blocking (LPB) ELISA was examined, and the mean 'r' value was 0.62 relative to vaccine virus IND 63/72. Some of the field isolates (n = 34) were tested in virus neutralization test (VNT) and showed an 'r' value of >0.40. Although a minor antigenic difference was observed in the Mab profiling study, there has not been large antigenic divergence between reference virus and field viruses, thereby providing evidence of wide antigenic coverage of the vaccine strain.
使用一组11种单克隆抗体（Mab）通过夹心ELISA对100株亚洲1型口蹄疫病毒（FMDV）田间分离株进行了比较。大多数（超过89%）分离株对Mab 2A、13、40、34和81显示出同源性（反应性76%及以上）或亲和力降低（反应性20 - 75%），这表明这些Mab结合表位是保守的，而对于Mab B3、1A、24、72、82和89观察到更具变异性的反应性。检测了液相阻断（LPB）ELISA中田间分离株的多克隆关系（“r”值），相对于疫苗病毒IND 63/72，平均“r”值为0.62。对一些田间分离株（n = 34）进行了病毒中和试验（VNT），显示“r”值>0.40。尽管在Mab分析研究中观察到了微小的抗原差异，但参考病毒和田间病毒之间尚未出现大的抗原分歧，从而为疫苗株的广泛抗原覆盖提供了证据。
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