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基于聚合酶链反应的淋巴丝虫病检测

Polymerase chain reaction-based detection of lymphatic filariasis.

作者信息

Fischer Peter, Boakye Daniel, Hamburger Joseph

机构信息

Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.

出版信息

Med Microbiol Immunol. 2003 Feb;192(1):3-7. doi: 10.1007/s00430-002-0152-z. Epub 2002 Oct 19.

Abstract

PCR-based diagnostic assays are promising tools for the monitoring and evaluation of the Global Programme for Elimination of Lymphatic Filariasis. Sensitive and specific assays have been described for the detection of Wuchereria bancrofti, Brugia malayi, or Brugia timori infection in blood, sputum, and vectors. These techniques can be most cost-effective when employed for pool screening, which is important in the later stages of control programs when infection rates of humans and vectors are low, and large numbers of samples must be examined.

摘要

基于聚合酶链反应(PCR)的诊断检测方法是全球消除淋巴丝虫病规划监测和评估的有前景的工具。已描述了用于检测血液、痰液和病媒中班氏吴策线虫、马来布鲁线虫或帝汶布鲁线虫感染的灵敏且特异的检测方法。当用于混合样本筛查时,这些技术可能最具成本效益,这在控制项目后期很重要,因为那时人和病媒的感染率较低,必须检测大量样本。

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