Abdel-Shafi Iman R, Shoieb Eman Y, Attia Samar S, Rubio José M, Ta-Tang Thuy-Huong, El-Badry Ayman A
Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.
Malaria & Emerging Parasitic Diseases Laboratory (MAPELab), National Microbiology Center, Instituto De Salud Carlos III, Madrid, Spain.
Parasitol Res. 2017 Mar;116(3):963-970. doi: 10.1007/s00436-017-5372-7. Epub 2017 Jan 24.
Lymphatic filariasis (LF) is a serious vector-borne health problem, and Wuchereria bancrofti (W.b) is the major cause of LF worldwide and is focally endemic in Egypt. Identification of filarial infection using traditional morphologic and immunological criteria can be difficult and lead to misdiagnosis. The aim of the present study was molecular detection of W.b in residents in endemic areas in Egypt, sequence variance analysis, and phylogenetic analysis of W.b DNA. Collected blood samples from residents in filariasis endemic areas in five governorates were subjected to semi-nested PCR targeting repeated DNA sequence, for detection of W.b DNA. PCR products were sequenced; subsequently, a phylogenetic analysis of the obtained sequences was performed. Out of 300 blood samples, W.b DNA was identified in 48 (16%). Sequencing analysis confirmed PCR results identifying only W.b species. Sequence alignment and phylogenetic analysis indicated genetically distinct clusters of W.b among the study population. Study results demonstrated that the semi-nested PCR proved to be an effective diagnostic tool for accurate and rapid detection of W.b infections in nano-epidemics and is applicable for samples collected in the daytime as well as the night time. PCR products sequencing and phylogenitic analysis revealed three different nucleotide sequences variants. Further genetic studies of W.b in Egypt and other endemic areas are needed to distinguish related strains and the various ecological as well as drug effects exerted on them to support W.b elimination.
淋巴丝虫病（LF）是一个严重的媒介传播健康问题，班氏吴策线虫（W.b）是全球LF的主要病因，在埃及呈局部流行。使用传统的形态学和免疫学标准来鉴定丝虫感染可能具有难度，并会导致误诊。本研究的目的是对埃及流行地区居民体内的W.b进行分子检测、W.b DNA的序列变异分析和系统发育分析。从五个省份丝虫病流行地区的居民采集血液样本，采用针对重复DNA序列的半巢式PCR检测W.b DNA。对PCR产物进行测序；随后，对获得的序列进行系统发育分析。在300份血液样本中，有48份（16%）检测到W.b DNA。测序分析证实了PCR结果，仅鉴定出W.b物种。序列比对和系统发育分析表明，研究人群中的W.b存在基因上不同的聚类。研究结果表明，半巢式PCR被证明是一种有效的诊断工具，可准确快速地检测纳米流行中的W.b感染，适用于白天和夜间采集的样本。PCR产物测序和系统发育分析揭示了三种不同的核苷酸序列变体。需要对埃及和其他流行地区的W.b进行进一步的遗传学研究，以区分相关菌株以及对它们产生的各种生态和药物影响，以支持消除W.b。
