Oyewole S H, Holt S C
Arch Microbiol. 1976 Mar 19;107(2):167-82. doi: 10.1007/BF00446836.
The lamellar membrane stacks of Ectothiorhodospira mobilis were isolated and purified by a combination of lysozyme and osmotic shock treatment, followed by differential and density gradient centrifugation. Preparations of lamellar membranes were enriched at least 2.4-fold in the ratio of bacteriochlorophyll a to protein. Thin-sectioning, negative staining, platinum-carbon shadowing and freeze-etching were used to study the architecture of the membrane units. Both platinum-carbon shadowing and freeze-etching showed the outer-surfaces of the isolated lamellar membrane stacks to be relatively smooth. Particles averaging 7 nm in diameter were seen on several faces following freeze-etching.
通过溶菌酶和渗透压休克处理相结合，随后进行差速离心和密度梯度离心，分离并纯化了运动外硫红螺菌的片层膜堆叠。片层膜制剂中细菌叶绿素a与蛋白质的比例至少富集了2.4倍。采用超薄切片、负染色、铂-碳复型和冷冻蚀刻技术研究膜单元的结构。铂-碳复型和冷冻蚀刻均显示，分离出的片层膜堆叠的外表面相对光滑。冷冻蚀刻后在几个面上可见平均直径为7nm的颗粒。
