Arimitsu Hideyuki, Inoue Kaoru, Sakaguchi Yoshihiko, Lee Jaechul, Fujinaga Yukako, Watanabe Toshihiro, Ohyama Tohru, Hirst Robert, Oguma Keiji
Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan.
Infect Immun. 2003 Mar;71(3):1599-603. doi: 10.1128/IAI.71.3.1599-1603.2003.
Clostridium botulinum serotype B toxins 12S and 16S were separated by using a beta-lactose gel column at pH 6.0; toxin 12S passed through the column, whereas toxin 16S bound to the column and eluted with lactose. The fully activated neurotoxin was obtained by applying the trypsin-treated 16S toxin on the same column at pH 8.0; the neurotoxin passed through the column, whereas remaining nontoxic components bound to the column. The toxicity of this purified fully activated neurotoxin was retained for a long period by addition of albumin in the preparation.
通过在pH 6.0条件下使用β-乳糖凝胶柱分离肉毒杆菌B型12S和16S毒素；12S毒素通过柱子，而16S毒素与柱子结合并随乳糖洗脱。将经胰蛋白酶处理的16S毒素在pH 8.0条件下应用于同一柱子，从而获得完全活化的神经毒素；神经毒素通过柱子，而其余无毒成分与柱子结合。通过在制剂中添加白蛋白，这种纯化的完全活化神经毒素的毒性可长期保留。
